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pACYCDuet_F31YL54IDHFR_WTTS

Plasmid

#91231

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_R166QTS

Plasmid

#91232

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_L54IDHFR_R166QTS

Plasmid

#91234

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_L54IDHFR_WTTS

Plasmid

#91235

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FDHFR_R166QTS

Plasmid

#91236

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPLtetO-PdhR

Plasmid

#89655

Purpose

Plasmid for titrating synthesis of PdhR with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

Pyruvate dehydrogenase complex repressor

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsB

Plasmid

#107559

Purpose

Plasmid for titrating synthesis of FtsB with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsB

Use

Synthetic Biology

Promoter

pLtetO

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S137D (#628)

Plasmid

#99944

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S137D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A

Plasmid

#98677

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_del1961-1970

Plasmid

#98680

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

del1961-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S103W (#623)

Plasmid

#99941

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S103W

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Epsilon (JCE592)

Plasmid

#89776

Purpose

yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Epsilon (Prkce Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-MAGE-112-429

Plasmid

#89128

Purpose

mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only