We narrowed to 4,673 results for: pcr
-
Plasmid#213231PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRD5 (DRD5 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR3-DuET
Plasmid#213199PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR8-DuET
Plasmid#213383PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR9-DuET
Plasmid#213384PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
S1PR1-DuET
Plasmid#213374PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterEFSAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP2-NbV5
Plasmid#201478PurposeBRET2 detection of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP2-NbV5
TagsGFP2ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX FLAG-SLC38A9.1
Plasmid#199749PurposeLentiviral construct to stably express SLC38A91.1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLC38A9.1 (SLC38A9 Human)
TagsFlagExpressionMammalianPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL472
Plasmid#69882PurposedCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassetteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIRL (Cirl Fly)
UseTransformation vectorTagsFLAGAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA8
Plasmid#158486PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorMutationL17E, D481V, STOP codonAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PK1-Tango
Plasmid#66478PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPK1
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
NMUR2-DuET
Plasmid#213350PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNMUR2 (NMUR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR77-DuET
Plasmid#213291PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR77 (C5AR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FFA2-DuET
Plasmid#213238PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFFA2 (FFAR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) WT-myc
Plasmid#199684PurposeTransient expression of LYCHOS (GPR155) WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR155 (GPR155 Human)
TagsmycExpressionMammalianPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) FP>IA-HA
Plasmid#199661PurposeTransient expression of LYCHOS (GPR155) FP>IA mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR155 (GPR155 Human)
TagsHAExpressionMammalianMutationF43 and P44 mutated to I and APromoterCMVAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-2
Plasmid#109009PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMC4-mAID-HaloTag (SMC4 Human)
ExpressionBacterial and MammalianAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only