We narrowed to 22,191 results for: his

1. pETM33_SIN3A_PAH

   Plasmid

   #178486

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SIN3A_PAH (SIN3A Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 Orai1

   Plasmid

   #21638

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a - Ec-ClpX

   Plasmid

   #111516

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJL1-sfGFP-DQNAT

   Plasmid

   #198210

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_DQNAT
   

   Use

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGM202T7

   Plasmid

   #69993

   Purpose

   E. coli/Streptomyces expression vector containing the T7-promoter

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aphII
   tsr
   rep
   sso
   T7 promoter
   dso
   His-Orf
   

   Use

   Tags

   N-terminal and C-terminal His tag encoding sequen…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AF1521-c002

   Plasmid

   #173109

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTDpelB-C_sfYFPTwinStrep

   Plasmid

   #45944

   Purpose

   expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Insert

   synthetic sfYFP (codon usage adapted to P.putida KT2440)
   

   Use

   Tags

   PelB export sequence and Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq/Ptrc

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AF1521-c003

   Plasmid

   #173111

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. APLF-c001

   Plasmid

   #173077

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   APLF (APLF Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K360-V448, codon optimized

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEThT-SAHS1

    Plasmid

    #90025

    Purpose

    To express His-tagged SAHS1 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ARH1-c003

    Plasmid

    #173093

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRH (ADPRH Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-L357, codon optimized

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MacroH2A.2-c002

    Plasmid

    #173089

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A2 (MACROH2A2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K167-K372

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJCC_076 AsCas12a R1226A

    Plasmid

    #171669

    Purpose

    For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a R1226A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginine 1226 to alanine

    Promoter

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLN382 (FuGW-S(HIF1A)p-mKate2 )

    Plasmid

    #105180

    Purpose

    synthetic promoter HIF1A drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(HIF1A)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEThT-CAHS1

    Plasmid

    #90027

    Purpose

    To express His-tagged CAHS1 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLN592 (FuGW-S(USF1)p-mKate2)

    Plasmid

    #105204

    Purpose

    synthetic promoter USF1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(USF1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ALC1-c007

    Plasmid

    #173112

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-MRPP1

    Plasmid

    #67862

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLN379 (FuGW-S(E2F1)p-mKate2)

    Plasmid

    #105177

    Purpose

    synthetic promoter E2F1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDDGFP2_leu2d_NRT1.1

    Plasmid

    #58332

    Purpose

    Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

    Insert

    NRT1.1 (NRT1.1 Mustard Weed)
    

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLN599 (pFuGW-hUbCp-hSTE1)

    Plasmid

    #105206

    Purpose

    ubiquitin C promoter drives STE expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    hUbCp-hSTE1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLN377 (FuGW-S(SP1)p-mKate2 )

    Plasmid

    #105176

    Purpose

    synthetic promoter SP1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(SP1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM33_PEX14_Pex14

    Plasmid

    #178484

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PEX14_Pex14 (PEX14 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA528 SPASTIN , residues 108-200

    Plasmid

    #180628

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3.1(-) + SLNCR1

    Plasmid

    #86816

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138734

    Purpose

    Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLN466 (FuGW-S(cMYC)p-mKate2)

    Plasmid

    #105189

    Purpose

    synthetic promoter cMYC drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(cMYC)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLN351 (FuGW-S(YBX1)p-mKate2 )

    Plasmid

    #105174

    Purpose

    synthetic promoter YBX1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(YBX1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pETSUMO-TtAgocatdead

    Plasmid

    #210322

    Purpose

    Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Use

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLN352 (FuGW-S(Elk1)p-mKate2)

    Plasmid

    #105175

    Purpose

    synthetic promoter Elk1 drives mKate2 experssion

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(Elk1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-hDcp2 E147Q, E148Q

    Plasmid

    #72215

    Purpose

    bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E147Q, E148Q

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pLN381 (FuGW-S(NFY)p-mKate2)

    Plasmid

    #105179

    Purpose

    synthetic promoter NFY drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(NFY)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCellFree_G03 STAT3

    Plasmid

    #67120

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only