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pET11a-N-DiB2

Plasmid

#168472

Purpose

N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1127

Plasmid

#160859

Purpose

PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Acetyl CoA geraniol/citronellol acetyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab38a

Plasmid

#109699

Purpose

Multisite gateway vector for 3' tagging with rab38a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab38a (rab38a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-3'UTR

Plasmid

#136042

Purpose

UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-CDS

Plasmid

#136043

Purpose

UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.040

Plasmid

#119584

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23110MH

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF8_isoD_CRISPR_resistant

Plasmid

#122468

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Mutation

Synonymous mutations conferring resistance to gRN…

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway cloning

Mutation

Synonymous mutations conferring resistance to gRN…

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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env8 16nt J1/13

Plasmid

#99832

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 16nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 16 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 19nt J1/13

Plasmid

#99834

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 19nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 19 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 17nt J1/13

Plasmid

#99833

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 17nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 17 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 24nt J1/13

Plasmid

#99835

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 24nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 24 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 7U J1/13

Plasmid

#99829

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 7U J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to 7 U's

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 AC rep J1/13

Plasmid

#99830

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 AC rep J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to repeating AC's

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 25nt J1/13

Plasmid

#99836

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 25nt J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 25 nucleotides

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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