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pD649-HAsp-IFNG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156535

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IFNG (IFNG Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC10-Fc(DAPA)-AviTag-6xHis

Plasmid

#156906

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC10 (SIGLEC10 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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DBDmut-CA-RIT-NFAT1

Plasmid

#63669

Purpose

Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding

Depositor

Anjana Rao

Article

Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

Insert

NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

3x HA

Expression

Mammalian

Mutation

CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
Anti-GABA-A-R-Pi [N431/64R]

Plasmid

#206576

Purpose

Mammalian Expression Plasmid of anti-GABA-A-R-Pi (Human). Derived from hybridoma N431/64.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA-A-R-Pi (Homo sapiens) recombinant Mouse monoclonal antibody (GABRP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_BRD9_NUTM1

Plasmid

#205783

Purpose

Express mEGFP-tagged fusion protein, BRD9_NUTM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

BRD9_NUTM1 (BRD9, NUTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156668

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF10B-Fc(DAPA)-AviTag-6xHis

Plasmid

#156546

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF10B (TNFRSF10B Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PTK7-Fc(DAPA)-AviTag-6xHis

Plasmid

#156943

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTK7 (PTK7 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD8_STOP

Plasmid

#161048

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD8 (MFSD8 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF1687

Plasmid

#143836

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSPA5_WT

Plasmid

#82132

Purpose

Gateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSPA5 (HSPA5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MKK1

Plasmid

#107973

Purpose

expression of MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 (MAP2K1 Human)

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRB1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156862

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRB1 (LILRB1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

Plasmid

#204696

Purpose

This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of the amino acids 355-505 of NUT

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

Plasmid

#204695

Purpose

This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(MIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of majority of NUT, retaining only amino…

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRB2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156863

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRB2 (LILRB2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

Plasmid

#156982

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTGFRN (PTGFRN Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC9-Fc(DAPA)-AviTag-6xHis

Plasmid

#156771

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC9 (SIGLEC9 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CD22-Fc(DAPA)-AviTag-6xHis

Plasmid

#156940

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD22 (CD22 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge