We narrowed to 29,768 results for: Ide

1. pLL2 sgRNA(MS2)-MS2-AID3c

   Plasmid

   #112128

   Purpose

   empty sgRNA cloning vector with MS2-AID3c

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono 3c mutant (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ206

   Plasmid

   #162679

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E226T; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ209

   Plasmid

   #162682

   Purpose

   Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of G251-T472 with W185-F191 from PETa…

   Promoter

   T7/lac

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-kappa-HA-dL5-BKa

   Plasmid

   #73212

   Purpose

   Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

   Insert

   BKalpha (Kcnma1 Mouse)
   

   Use

   Tags

   dL5**

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-ARAP3(R307,8A)

   Plasmid

   #39486

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3(R307,8A) (ARAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R307,8A = PH domain mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-ARAP3(C504A)

   Plasmid

   #39485

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3(C504A) (ARAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C504A = Arf GAP domain mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

   Plasmid

   #227608

   Purpose

   Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   aa 2-83 deleted

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCJ220

    Plasmid

    #162686

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224W, C529S; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCJ221

    Plasmid

    #162687

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224H, C529F; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid

    #227609

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-544, 651-757 only [TM deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid

    #227611

    Purpose

    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    MBP, 10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-88 only

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid

    #227610

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 2-400, 706-757 [TM and HB2 deleted]

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 E2 LBD-Tev-E1a-His

    Plasmid

    #232117

    Purpose

    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    DBT (DBT Human)
    

    Use

    Tags

    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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