We narrowed to 30,451 results for: Ide

1. pcDNA3.1-BKa

   Plasmid

   #73213

   Purpose

   Expresses BKa (KCNMA1, mSlo alpha subunit) in mammalian cells. (tagless, leaderless)

   Depositor

   Marcel Bruchez

   Article

   Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

   Insert

   BKalpha (Kcnma1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp16

   Plasmid

   #156482

   Purpose

   Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Circular 200,100 (GAPDH)

   Plasmid

   #170120

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM33_Nsp9

   Plasmid

   #156478

   Purpose

   Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp9 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BE3-P2A-EGFP (pJUL977)

   Plasmid

   #123612

   Purpose

   CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_Nsp3d_Ub2_PLPro

   Plasmid

   #156472

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_Nsp1

    Plasmid

    #156465

    Purpose

    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCCL-PGK-SPdCas9-BFP-DNMT3A

    Plasmid

    #66819

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
    

    Tags

    dSpCas9-BFP-DNMT3A

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM33_N_CTD

    Plasmid

    #156484

    Purpose

    Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    N_CTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM33_N_NTD

    Plasmid

    #156483

    Purpose

    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    N_NTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p-miR-Report + WT miR-181 BS in GATA6 3UTR

    Plasmid

    #22794

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. p-miR-Report + MT miR-181 BS in GATA6 3UTR

    Plasmid

    #22795

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid

    #176589

    Purpose

    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Chesapeake Bay) locus
    

    Expression

    Bacterial

    Mutation

    Truncated CRISPR array to two direct repeats, rep…

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid

    #123616

    Purpose

    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    NLS-nCas9-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM33_Nsp7

    Plasmid

    #156476

    Purpose

    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM33_Nsp8

    Plasmid

    #156477

    Purpose

    Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM33_Nsp3b_ADRP

    Plasmid

    #156468

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3b_ADRP (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge