We narrowed to 165,385 results for: addgene

1. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15 PemrR-gfp biosensor

   Plasmid

   #185793

   Purpose

   The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

   Depositor

   Steven Hallam

   Article

   Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

   Insert

   pEmrR-gfp
   

   Tags

   EGFP

   Expression

   Bacterial

   Promoter

   PemrR

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJF144_SECGFP_sg2

   Plasmid

   #163865

   Purpose

   sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting GFP
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   R07E5.16 (U6)

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJF143_SECGFP_sg1

   Plasmid

   #163864

   Purpose

   sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting GFP
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   R07E5.16 (U6)

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMVTCRb_candidate8

   Plasmid

   #165006

   Purpose

   Expression of candidate TCR #8 TCRbeta cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   TRB (TRB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMVTCRa_candidate8

   Plasmid

   #165005

   Purpose

   Expression of candidate TCR #8 TCRalpha cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   TRAV (TRA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. RIB VP64 R2

   Plasmid

   #161494

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R2 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. RIB VP64 R3

   Plasmid

   #161495

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R3 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RIB VP64 R4

   Plasmid

   #161496

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R4 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Rab37

    Plasmid

    #140886

    Purpose

    Expression of zebrafish GFP-tagged Rab37 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab37
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Synapsin-mScarlet-C1-WPRE

    Plasmid

    #159955

    Purpose

    untargeted marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mScarlet-C1
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2 8xSTAR-sTomato. PGK-puro

    Plasmid

    #136264

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1xHRE-MinTK-CRE

    Plasmid

    #141142

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system.

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    1xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xHRE-MinTK-CRE

    Plasmid

    #141143

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    3xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDK134

    Plasmid

    #134187

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 aa 69 - 479 (knl-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM136

    Plasmid

    #134177

    Purpose

    Bacterial expression of his-CENP-C 700-943

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 700-943 (CENPC Human)
    

    Expression

    Bacterial

    Mutation

    aa 700-943

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUP95Q15AL460PGW (E3)

    Plasmid

    #74033

    Purpose

    lentiviral expression of Psd95-Q15A L460P-EGFP fusion

    Depositor

    Oliver Schluter, Weifeng Xu, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q15A, L460P

    Promoter

    hUbC

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

18. FUP95DPESTGW (C7)

    Plasmid

    #74023

    Purpose

    lentiviral expression of Psd95-DPEST-EGFP fusion

    Depositor

    Oliver Schluter, Weifeng Xu, Robert Malenka

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    DPEST; amino acids 10-25 deleted

    Promoter

    hUbC

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pEN_TT 3xFLAG LacZ

    Plasmid

    #83093

    Purpose

    Gateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Promoter

    TRE tight promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

20. p236_LTJ_sgRNAFoxp3sf/J

    Plasmid

    #82676

    Purpose

    sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 sf/J
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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