We narrowed to 6,987 results for: ef1a

1. pLKO_NWS.V5.FLAG_NGFR

   Plasmid

   #158251

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.V5.FLAG

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MLM3727

   Plasmid

   #49961

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CD HB-5 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLKO_S.V5.HA_NGFR

   Plasmid

   #158339

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   S.V5.HA

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p23-mNeongreen-DDX21 HGV

   Plasmid

   #179230

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Mammalian

   Mutation

   D338H, E339G

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p23-mNeongreen-DDX21 DAT

   Plasmid

   #179229

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Mammalian

   Mutation

   G234D, K235A

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBbi-Pur-cpVenus[E172]

   Plasmid

   #125202

   Purpose

   SB-transposon plasmid for stable expression of cpVenus[E172] cDNA as a FRET single color control

   Depositor

   Eric Davis

   Article

   Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

   Insert

   cpVenus[E172]
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VC388

   Plasmid

   #49958

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CD HB-4 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLKO_Ollas.AU1.C_NGFR

   Plasmid

   #158314

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.AU1.C

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO_Ollas.C.VSVg_NGFR

   Plasmid

   #158315

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.C.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO_Tag.C.S.Ollas_NGFR

    Plasmid

    #158303

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Tag.C.S.Ollas

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO_HSV.Ollas.C_NGFR

    Plasmid

    #158268

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HSV.Ollas.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

    Plasmid

    #51247

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. SL357

    Plasmid

    #49936

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1FL KL-1 (KLF4 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLKO_HA.AU1.V5_NGFR

    Plasmid

    #158235

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.AU1.V5

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_Ollas.C.V5_NGFR

    Plasmid

    #158310

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.V5

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_Ollas.C.FLAG_NGFR

    Plasmid

    #158311

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_Ollas.C.NWS_NGFR

    Plasmid

    #158312

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.NWS

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_Ollas.C.HA_NGFR

    Plasmid

    #158313

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.C.HA

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_HA.VSVg.C_NGFR

    Plasmid

    #158263

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.VSVg.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_FLAG.VSVg.C_NGFR

    Plasmid

    #158260

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG.VSVg.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag‐mTim3 (T2 truncation)

    Plasmid

    #49211

    Purpose

    expresses Flag tagged mouse Tim3 (T2 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T2 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T2 truncation ( lacks all but one tyrosine, which…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

    Plasmid

    #125204

    Purpose

    SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    mCerulean3-spacer-cpVenus[E172]
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JA1246

    Plasmid

    #49960

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-5 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

25. JA1238

    Plasmid

    #49957

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-4 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

26. JA431

    Plasmid

    #49937

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD KL-2 (KLF4 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

27. MLM3733

    Plasmid

    #49964

    Purpose

    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CD HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

28. JA1261

    Plasmid

    #49963

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

29. JA1290

    Plasmid

    #49968

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-9 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

30. JA1252

    Plasmid

    #49956

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-3 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only