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Showing: 3121 - 3140 of 27274 results

  5. pBI121::chitEclipGFP:LAAQ

    Plasmid
    #240452
    Purpose
    Expression of indicator protein fusion (modified ecliptic pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of Chitinase signal, soluble modified ecliptic pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240452-plasmid-map-sequence-id-485324

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  6. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  10. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170348-plasmid-map-sequence-id-336137

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  11. pZ:F3-P CAGGS tdTPH-F

    Plasmid
    #112667
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Expression
    Mammalian
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    112667-plasmid-map-sequence-id-229330

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  15. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  17. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  19. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid
    #115518
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    eGFP
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    115518-plasmid-map-sequence-id-229325

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Showing: 3121 - 3140 of 27274 results