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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Use

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1

Plasmid

#48970

Purpose

Expresses catalytic domain of MALT1 in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-TAF4(827-909)

Plasmid

#105620

Purpose

bacterially express TAF4 HFD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4-HFD(827-909) (Taf4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pET21a-mAIM2-deltaPYD-GST

Plasmid

#51534

Purpose

Express GST-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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