We narrowed to 30,451 results for: Ide

1. p35S_GFP-EVDinter-GUS

   Plasmid

   #167123

   Purpose

   Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
   

   Tags

   GUS and mGFP5

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp3e_NAB

   Plasmid

   #156473

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA

   Plasmid

   #139861

   Purpose

   Lentiviral expression of TOM20MTS-DDRGK1-dTM-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   TOM20-MTS-DDRGK1-dTM (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   recombinant fusion of mitochondria-targeting sign…

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

   Plasmid

   #128698

   Purpose

   simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

   Inserts

   iqgC (full length)
   Raf1 kinase (aa 50-132)
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP and mRFP

   Mutation

   none

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL5 sgRNA(MS2)-MS2-hA3a D131A

   Plasmid

   #112131

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131A

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131A mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131A mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs GFP-LC3-RFP

   Plasmid

   #117413

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

   Plasmid

   #128605

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   24xSunTag
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. C1ORF43-3xFLAG

    Plasmid

    #130880

    Purpose

    Expresses human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. C1ORF43(1-41)-3xFLAG

    Plasmid

    #130881

    Purpose

    Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 44-253

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-ccdB-boxB-tBE-V5-mA3

    Plasmid

    #171693

    Purpose

    Expresses tBE-V5-mA3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and EF1a

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG303-Gal-PA50

    Plasmid

    #84906

    Purpose

    induce expression of dipeptide repeat PA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PA50 (C9orf72 Synthetic)
    

    Tags

    FLAG and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG303-Gal-GA50

    Plasmid

    #84907

    Purpose

    induce expression of dipeptide repeat GA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GA50 (C9orf72 Synthetic)
    

    Tags

    FLAG and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-ARAP3

    Plasmid

    #39484

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3 (ARAP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pAG303-Gal-PR50

    Plasmid

    #84905

    Purpose

    induce expression of dipeptide repeat PR in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PR50 (C9orf72 Synthetic)
    

    Tags

    FLAG and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG303-Gal-GR100

    Plasmid

    #84908

    Purpose

    induce expression of dipeptide repeat GR in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GR100 (C9orf72 Synthetic)
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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