We narrowed to 13,968 results for: SHI
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Plasmid#146473PurposeInsect Expression of DmHPat-del500-684DepositorInsertDmHPat-del500-684 (Patr-1 Fly)
ExpressionInsectMutationthree silent mutations compared to the sequence g…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB2AR-miRFP718nano
Plasmid#195751PurposeBeta-2-adrenergic receptor internally tagged with miRFP718nanoDepositorInsertB2AR-miRFP718nano
ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP718nano
Plasmid#195748PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertmiRFP718nano-LC-Myosin
TagsmiRFP718nanoExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP718nano
Plasmid#195743PurposeMonomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmidDepositorInsertmiRFP718nano
TagsHisExpressionBacterialPromoteraraCAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGas-miRFP718nano
Plasmid#195752PurposeG protein alpha-subunit internally tagged with miRFP718nanoDepositorInsertG protein alpha-s-miRFP718nano
ExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-VC155
Plasmid#194051PurposeTransient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-mVenus
Plasmid#194000PurposeTransient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)DepositorInsertmVenus
TagsAtOEP7 1–50aaExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-VN154
Plasmid#194050PurposeTransient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP7_rpoA-U
Plasmid#190974PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP7
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-E67A_rpoA-U
Plasmid#190959PurposeTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-C
Plasmid#190968PurposeTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP5_rpoA-U
Plasmid#190985PurposeTest the U-to-C editing activity of KP5 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP5
TagsHisExpressionMammalianPromoterCMVAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP1_rpoA-U
Plasmid#190955PurposeTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP1
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-A66S_rpoA-U
Plasmid#190957PurposeTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-A66S_rpoA-U
Plasmid#190961PurposeTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP7_rpoA-U
Plasmid#190994PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP7
TagsHisExpressionMammalianPromoterCMVAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-E67A_rpoA-U
Plasmid#190963PurposeTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP4_rpoA-U
Plasmid#190964PurposeTest the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP4
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-E67A_rpoA-C
Plasmid#190972PurposeTest the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP4_rpoA-U
Plasmid#190984PurposeTest the U-to-C editing activity of KP4 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP4
TagsHisExpressionMammalianPromoterCMVAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP5_rpoA-U
Plasmid#190965PurposeTest the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP5
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-CM
Plasmid#188585PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler Catalytic mutant
TagsFLAG-MycExpressionInsectMutationdeleted first ATG; D435A [GAT=>GCC]; E435A [GA…Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[N1-383]
Plasmid#188586PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler N-terminal domain
TagsFLAG-MycExpressionInsectMutationdeleted first ATGAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]
Plasmid#188587PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain
TagsFLAG-MycExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-1xL
Plasmid#186911PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)1-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-AA027
Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N3-myc-SLC35A2
Plasmid#186285Purposetransient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
gSCPL35-mRFP
Plasmid#181960PurposeSCPL35 protein tagged with mRFP, under control of native promoterDepositorInsertSCPL35
TagsmRFPExpressionPlantPromoternative SCPL35Available SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-499_B
Plasmid#145979PurposeInsect Expression of DmHPat_57-499DepositorInsertDmHPat_57-499 (Patr-1 Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_1-267_D
Plasmid#146118PurposeInsect Expression of DmMe31B_1-267DepositorInsertDmMe31B_1-267 (me31B Fly)
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_1-225_A
Plasmid#145870PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
ExpressionInsectMutationone non silent mutation N218K compared to the seq…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_500-968_A
Plasmid#145873PurposeInsect Expression of DmHPat_500-968DepositorInsertDmHPat_500-968 (Patr-1 Fly)
ExpressionInsectMutationone silent mutation A1758G compared to the sequen…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-225_A
Plasmid#145875PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
ExpressionInsectMutationone non silent mutation N218K compared to the seq…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-499_A
Plasmid#145876PurposeInsect Expression of DmHPat_1-499DepositorInsertDmHPat_1-499 (Patr-1 Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmXRN1_A
Plasmid#145879PurposeInsect Expression of DmXRN1DepositorInsertDmXRN1 (pcm Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY282F
Plasmid#172468Purposeexpresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domainDepositorInsertwbp2nlY282F
UseXenopus expressionTagsnoneMutationchanged Tyrosine at aa282 to PhenylalaninePromoterSP6Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_Cap
Plasmid#175797Purposede novo thick circular tandem repeat protein capped to prevent multimerizationDepositorInserttcTRP24sub8_Cap
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SH2-free
Plasmid#175800PurposeHuman Nck2 SH2 domainDepositorInserthuman Nck2 SH2
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP9
Plasmid#175793Purposede novo thick circular tandem repeat proteins with 9 repeatsDepositorInserttcTRP9
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlT45A
Plasmid#172464Purposeexpresses protein with one aa substitution to affect potential phosphorylationDepositorInsertwbp2nlT45A
UseXenopus expressionTagsnoneMutationchanged Threonine at aa45 to AlaninePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY55F
Plasmid#172466Purposeexpresses protein with one aa substitution to affect potential phosphorylation in ITSM domainDepositorInsertwbp2nlY55F
UseXenopus expressionTagsnoneMutationchanged Tyrosine at aa55 to PhenylalaninePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585-gp130
Plasmid#174404PurposeBacterial expression of sarA-gp130 chimera under native promoter.DepositorInsertsarA-IL6ST chimera
ExpressionBacterialPromoterEndogenousAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyExpressionMammalianPromoterCMV-tetO2Available SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_268-459_D
Plasmid#146119PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
ExpressionInsectAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB-RM
Plasmid#168476PurposeN-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertN-fragment of DiB-RM
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB2
Plasmid#168473PurposeN-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip proteinDepositorInsertN-fragment of DiB2 + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only