We narrowed to 3,227 results for: RFP

1. pcDNA3-RAB-EKARev-NLS

   Plasmid

   #118476

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev-NLS
   

   Tags

   6xHIS, Nuclear localization signal (NLS), T7 tag …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3111 (pSpy1C-Plac(3)_ffluc)

   Plasmid

   #218778

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(3)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.Active/Repressed TLR (Sce)

   Plasmid

   #45575

   Purpose

   Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   eGFP with I-Sce I TS
   +3 mCherry
   

   Use

   Lentiviral

   Tags

   T2A dislinker and iRFP

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-DrFLINC-AKAR-Kras

   Plasmid

   #182334

   Purpose

   Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DrFLINC-AKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Expression

   Mammalian

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNSSCPG

   Plasmid

   #183187

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   superfolder GFP
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1617 pAAV SYN1 Nuc-EYFP

   Plasmid

   #135567

   Purpose

   An AAV vector expressing a neuronally expressed nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   empty
   Nuc-EYFP
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   hSYN1

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W

   Plasmid

   #126522

   Purpose

   Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.

   Depositor

   Rusty Lansford

   Article

   Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. d

   Insert

   H2B-CeruleanFP-2A-Dendra2FP
   

   Use

   Lentiviral

   Promoter

   hUbC

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAN357

    Plasmid

    #120898

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN460

    Plasmid

    #137878

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol1 - 14xUAS:CaSR-EGFP

    Plasmid

    #191502

    Purpose

    A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol1 transgenes…

    Promoter

    14X UAS

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGA-red-mini

    Plasmid

    #196338

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGA-red-maxi

    Plasmid

    #196337

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DsRed -7e

    Plasmid

    #191765

    Purpose

    Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Tags

    N terminal fusion of negatively charged polypetod…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. RGB-S reporter

    Plasmid

    #207841

    Purpose

    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

    Depositor

    Christof Niemeyer

    Article

    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

    Inserts

    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    

    Expression

    Bacterial

    Promoter

    PosmY, PsulA, and grpE

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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