We narrowed to 253 results for: T7 rna polymerase

1. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIA1363

   Plasmid

   #166861

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp8 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIA1362

   Plasmid

   #166860

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp7 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. C98m

   Plasmid

   #121002

   Purpose

   MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Gp2 toxin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pARiBo1

   Plasmid

   #98892

   Purpose

   Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

   Depositor

   Pascale Legault

   Article

   Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

   Insert

   ARiBo1
   

   Use

   In vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15bLCFPT7

   Plasmid

   #53545

   Purpose

   Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

   Depositor

   Lingchong You

   Article

   Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

   Insert

   T7 RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PVS10

    Plasmid

    #104398

    Purpose

    polycistronic vector for expression of E. coli core RNAP

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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