We narrowed to 407 results for: ta cloning vector

1. pMSR:MSMEG_0709:Dendra

   Plasmid

   #159077

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0709
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-3xFLAG-TAF5-∆ex8

   Plasmid

   #209050

   Purpose

   Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-miniTurbo-TAF5-∆ex8

   Plasmid

   #209052

   Purpose

   Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-3xFLAG-TAF5-FL_WobbleMut

   Plasmid

   #209055

   Purpose

   Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

   Plasmid

   #209056

   Purpose

   Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-nSpCas9-NoCBE

   Plasmid

   #209038

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-nSpCas9-APOBEC1

   Plasmid

   #209039

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-nSpCas9-BEACON2

   Plasmid

   #209041

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   BEACON2-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-nSpCas9-TadA8.20m

   Plasmid

   #209045

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8.20m-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid

    #218973

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutant SRSF1 binding motif (AGTCTCCT)

    Promoter

    RSV LTR

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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