We narrowed to 505 results for: SPY

1. InterCatch-1xL

   Plasmid

   #186907

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Taekjip Ha, Allen Liu

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80l A-CC-A

   Plasmid

   #196478

   Purpose

   Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   A-CC-A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC-cUnaG

   Plasmid

   #207631

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSC-LPETG

   Plasmid

   #207629

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. InterTag-1xL

   Plasmid

   #186911

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

   Depositor

   Taekjip Ha, Allen Liu

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   SpyTag003-sfCherry11-(GGGGS)1-PDGFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ262m3

   Plasmid

   #149556

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC-cUnaG-LPETG

   Plasmid

   #207635

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ265H

   Plasmid

   #149554

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   hAID-ziSpyMacCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ266B

    Plasmid

    #149555

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ziSpyMacCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. M2e-noro-VLP

    Plasmid

    #201192

    Purpose

    Expresses noro-VLP decorated with influenza M2e in insect cells

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

    Insert

    M2e-noro-VLP
    

    Use

    Tags

    Influenza M2e peptide

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-MMLVgag-3xNES-Cas9

    Plasmid

    #181752

    Purpose

    MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLV-Gag-3xNES-Cas9 (Spy)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SUMO-KTag

    Plasmid

    #51723

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag

    Depositor

    Mark Howarth

    Article

    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

    Insert

    SUMO-KTag
    

    Use

    Tags

    His6 Tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pDEST14-DoubleCatcher-H-Lock

    Plasmid

    #216285

    Purpose

    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher H-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AcrIIA4

    Plasmid

    #101043

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA4
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge