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pPEE17

Plasmid

#128361

Purpose

Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Insert

Acetamidase (amdS)

Use

Unspecified

Tags

Expression

Mutation

Promoter

TEF1

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pstop

Plasmid

#109066

Purpose

inserts a polyA site to end transcription of a targetted gene

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Use

Tags

EGFP

Expression

Worm

Mutation

Promoter

dpy-7

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pPK348

Plasmid

#38145

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

ptc-3::GFP (ptc-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

ptc-3

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pET-11-LECT2-V40

Plasmid

#183031

Purpose

Expression of LECT2 V40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Use

Tags

Expression

Bacterial

Mutation

V40 variant

Promoter

T7

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

p-T5 Lac

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR Clover-VISH2

Plasmid

#188629

Purpose

Fluorescent reporter for SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

VISH2

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Expression

Mutation

Promoter

mouse CamKII alpha

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPK700

Plasmid

#38146

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

let-60p::ptc-3::gfp (ptc-3 Nematode)

Use

Tags

EGFP

Expression

Worm

Mutation

Promoter

let-60

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-P2A-EGFP

Plasmid

#216257

Purpose

ExSYTE (P2A) EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGW1-ExSYTE-EGFP

Plasmid

#216259

Purpose

ExSYTE - EGFP fusion

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-ExSYTE

Plasmid

#216265

Purpose

ExSYTE in oocyte vector

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE

Use

Crna synthesis for xenopus laevis oocyte

Tags

none

Expression

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pIN1_GGGSx3_mScarlet

Plasmid

#182936

Purpose

C-terminal mScarlet with flexible linker (GGGGS)x3

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564_UUCG-loop

Plasmid

#133581

Purpose

T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

P2-deletion mutant of Tte preQ1-riboswitch

Use

Unspecified

Tags

Expression

Mutation

replacement of riboswitch nucleotides U6 through …

Promoter

T7

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1 GW

Plasmid

#79750

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nat Genet. 2006 Jan;38(1):63-7. Epub 200

Type

Empty backbone

Use

Tags

none

Expression

Plant

Mutation

Promoter

ProCDKA;1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGWKS9

Plasmid

#139416

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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