We narrowed to 3,089 results for: sam

1. FGFR2 N549K 3xFlag

   Plasmid

   #110107

   Purpose

   expresses FGFR2 with a single amino acid change in mammalian cells

   Depositor

   Martin Fernandez-Zapico

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mutated Asparagine 549 to Lysine (N549K)

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FGFR2 L617V 3xFlag

   Plasmid

   #110110

   Purpose

   expresses FGFR2 with a single amino acid change in mammalian cells

   Depositor

   Martin Fernandez-Zapico

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mutated Leucine 617 to Valine (L617V)

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101102

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   TMt-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA and Myc

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101107

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPJ167(JPUB_006765)

   Plasmid

   #83831

   Purpose

   Carries NEW operon 3 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste2803
   Kuste2804
   

   Promoter

   op3_2804_for and op3_BsmBI_2803_for

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101115

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   LPAR1-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101113

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101111

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   AVPR2-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SuExp-mPLD3

    Plasmid

    #201245

    Purpose

    express full length mouse PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (Pld3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC_C_FGFR2

    Plasmid

    #187751

    Purpose

    MAC-tagged gene expression of human FGFR2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    FGFR2 (FGFR2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAct:MS2-p65-HSF

    Plasmid

    #78902

    Purpose

    Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-p65-HSF
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SuExp His-Myc-mPLD3

    Plasmid

    #173853

    Purpose

    Protein expression plasmid for recombinant His-Myc tag mouse PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (Pld3 Mouse)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC3-FGFR1

    Plasmid

    #222611

    Purpose

    Expression of MAC3-tagged FGFR1

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    FGFR1 (FGFR1 Human)
    

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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