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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,228 results for: sequence

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Showing: 3181 - 3200 of 13228 results

  1. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  2. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  3. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  4. pTRIPZ (M)-HT-NLS

    Plasmid
    #82518
    Purpose
    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Nuclear localizaition sequence
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82518-plasmid-map-sequence-id-156794

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  5. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Chang Liu
    Insert
    MET15
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213782-plasmid-map-sequence-id-424626

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  6. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    MIEPmouse CMV MIEP
    Available Since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

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  7. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  9. pPICZac-STE2-1D4

    Plasmid
    #133437
    Purpose
    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S/C252A
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133437-plasmid-map-sequence-id-267729

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  10. pENTR-R2L3-pA-floxPGKneopA

    Plasmid
    #139543
    Purpose
    Provides pA sequences N-terminal to floxed PGK-neo-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Neomycin resistant gene
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139543-plasmid-map-sequence-id-274621

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  11. pIC234

    Plasmid
    #69733
    Purpose
    Retroviral expression of Nup-37
    Depositor
    Iain Cheeseman
    Insert
    Nup-37 (NUP37 Human)
    Use
    Retroviral
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    E121K compared to GenBank reference sequence NP_0…
    Available Since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pIC215

    Plasmid
    #69736
    Purpose
    Mammalian Expression of PELP1
    Depositor
    Iain Cheeseman
    Insert
    PELP1 (PELP1 Human)
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    E927G compared to GenBank reference sequence NP_0…
    Available Since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. (P79) OCN/Wnt 10b transgene

    Plasmid
    #25878
    Depositor
    Ormond MacDougald
    Insert
    Wnt10b (Wnt10b Mouse)
    Use
    Mouse Targeting
    Tags
    B-globin intron/poly A coding sequence and OCN pr…
    Expression
    Mammalian
    Available Since
    Oct. 21, 2010
    Availability
    Academic Institutions and Nonprofits only

  14. pBID2_UAS>Gateway

    Plasmid
    #173723
    Purpose
    Drosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesis
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Mutation
    20 x UAS sequences
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    173723-plasmid-map-sequence-id-345256

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  15. pBID2_LexOp>Gateway

    Plasmid
    #173724
    Purpose
    Drosophila transgene expression under LexOp/LexA control, PhiC31 transgenesis
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Mutation
    13 x LexOp sequences
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    173724-plasmid-map-sequence-id-341679

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  16. pBID2_QUAS>Gateway

    Plasmid
    #173725
    Purpose
    Drosophila transgene expression under QUAS/QF control, PhiC31 transgenesis
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Mutation
    10 x QUAS sequences
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    173725-plasmid-map-sequence-id-341673

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  17. pBID2_MCS>LexA:QF

    Plasmid
    #194962
    Purpose
    Drosophila LexA:QF expression under introduced sequence control, PhiC31 transgenesis
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Expression
    Insect
    Mutation
    LexA DNA binding domain, QF activation domain
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    194962-plasmid-map-sequence-id-384162

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  18. pBID2_MCS>QF:Gal4

    Plasmid
    #194964
    Purpose
    Drosophila QF:Gal4 expression under introduced sequence control, PhiC31 transgenesis
    Depositor
    Brian McCabe
    Type
    Empty backbone
    Expression
    Insect
    Mutation
    QF DNA binding domain, GAL4 activation domain
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    194964-plasmid-map-sequence-id-384142

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  19. pCAGGS-MTS(S17A)-EGFP-V5

    Plasmid
    #200928
    Purpose
    expresses MTS(S17A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S17A in MTS
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200928-plasmid-map-sequence-id-409553

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  20. pCAGGS-MTS(S27A)-EGFP-V5

    Plasmid
    #200930
    Purpose
    expresses MTS(S27A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S27A in MTS
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200930-plasmid-map-sequence-id-409412

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Showing: 3181 - 3200 of 13228 results