We narrowed to 6,467 results for: poly

1. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIS1 Cyclin D2 long UTR

   Plasmid

   #21645

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 long UTR (CCND2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB5191

   Plasmid

   #126911

   Purpose

   Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   X-4 and XII-5 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE flag M33

   Plasmid

   #1951

   Depositor

   Robert Kingston

   Article

   Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

   Insert

   M33 (Cbx2 Mouse)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pSV(X) C/EBPbeta

   Plasmid

   #13393

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPbeta (Cebpb Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pMH-HT_CtCHAD

   Plasmid

   #127785

   Purpose

   Expresses CtCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. psicheck-2-Tas2r3

   Plasmid

   #48176

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of TAS2R3 (TAS2R3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pSV(X) C/EBPalpha

   Plasmid

   #13392

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPalpha (Cebpa Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pFastBac K-M33

   Plasmid

   #1986

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   M33 (Cbx2 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Kozak sequence added before ATG

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pJin-290

    Plasmid

    #125718

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPRIME-TET-GIN-recipient

    Plasmid

    #11656

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP, IRES, and Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCk1

    Plasmid

    #136695

    Purpose

    Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Psy074

    Plasmid

    #99504

    Purpose

    Bacterial expression of LIPS M1+TE

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    LIPS M1+TE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX156

    Plasmid

    #29482

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    Kif2A-beta (Kif2a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV5 MYC-Smurf2 (FF29/30A) C716A

    Plasmid

    #24605

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, C716A

    Promoter

    Available since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. BB-­NIC-­II-­HisN-­TET12

    Plasmid

    #45496

    Depositor

    Roman Jerala

    Article

    Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

    Insert

    TET12
    

    Use

    Synthetic Biology

    Tags

    6xHistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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