We narrowed to 10,458 results for: T7

1. pJK359

   Plasmid

   #71570

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   putative CoA-transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK278

   Plasmid

   #71134

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-89 to asparagine; additional N-…

   Promoter

   T7

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK130

   Plasmid

   #71129

   Purpose

   Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changed valine-319 to phenylalanine

   Promoter

   T7

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK286

   Plasmid

   #71567

   Purpose

   Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK358

   Plasmid

   #71569

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   putative CoA-transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK320

   Plasmid

   #71144

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to serine; additional N-term…

   Promoter

   T7

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK319

   Plasmid

   #71143

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to alanine; additional N-ter…

   Promoter

   T7

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK318

   Plasmid

   #71142

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to glutamine; additional N-t…

   Promoter

   T7

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK315

   Plasmid

   #71141

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-89 to glycine; additional N-ter…

   Promoter

   T7

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK314

    Plasmid

    #71140

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to valine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK279

    Plasmid

    #71135

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK283

    Plasmid

    #71139

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK282

    Plasmid

    #71138

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK281

    Plasmid

    #71137

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK280

    Plasmid

    #71136

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 IRF9

    Plasmid

    #67079

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCellFree_G03 BTF3L4

    Plasmid

    #67051

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3L4 (BTF3L4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET151-hUCH37 (ISF2)

    Plasmid

    #61930

    Purpose

    Expression of hUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDEST14-ARL4C

    Plasmid

    #67381

    Purpose

    Expression of native hArl4c in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL7 (ARL4C Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pDEST14-ARL8A

    Plasmid

    #67382

    Purpose

    Expression of native hArl8 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8A (ARL8A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pDEST14-ARF3

    Plasmid

    #67370

    Purpose

    Expression of native hArf3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF3 (ARF3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pDEST14-ARF4

    Plasmid

    #67371

    Purpose

    Expression of native hArf4 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pDEST14-ARF6

    Plasmid

    #67373

    Purpose

    Expression of native hArf6 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pDEST14-ARL2

    Plasmid

    #67375

    Purpose

    Expression of native hArl2 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL2 (ARL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pfMDH_Triple

    Plasmid

    #67733

    Purpose

    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine, arginine 373 to…

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pET15b+PCNA1 G108C/P186C

    Plasmid

    #66174

    Purpose

    Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C, P186C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7 cyclin A1-164 venus cks1 (2196)

    Plasmid

    #61839

    Purpose

    expression of N terminal cyclin A and cks1

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    L2V

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pUC19 gene 60 thru gap + Cys

    Plasmid

    #49161

    Purpose

    Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Use

    Tags

    Expression

    Mutation

    gene 60 mRNA truncated two codons after coding ga…

    Promoter

    T7

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. M26L DJ-1 pET15b

    Plasmid

    #60679

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pET-Ce25

    Plasmid

    #51296

    Purpose

    Expresses a plant arginyl-tRNA synthetase in E.coli

    Depositor

    Gabor Igloi

    Article

    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7

    Insert

    arginyl-tRNA synthetase
    

    Use

    Tags

    His Tag and Thioredoxin

    Expression

    Bacterial

    Mutation

    GTT (Val) insertion after initiation codon to per…

    Promoter

    T7

    Available since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Fast-GCaMP-RS09

    Plasmid

    #52649

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS09
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L414T mutated from GCaMP3

    Promoter

    T7

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pHL 1394

    Plasmid

    #53009

    Purpose

    pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    T7(st3) (133bps)
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

34. Fast-GCaMP-RS05

    Plasmid

    #52646

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS05
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M373Q mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

35. Fast-GCaMP-RS08

    Plasmid

    #52648

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS08
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T412Q/L414T mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

36. Danio rerio cryaa V62T

    Plasmid

    #47630

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 62 to Threonine

    Promoter

    T7

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Danio rerio cryaa T147V

    Plasmid

    #47628

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Theronine 147 to Valine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Danio rerio cryaa C143S

    Plasmid

    #47619

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 143 to Serine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TAL3589

    Plasmid

    #47308

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ybx1-right (ybx1 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

40. TAL3588

    Plasmid

    #47307

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ybx1-left (ybx1 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only