We narrowed to 39,996 results for: kan

1. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

   Plasmid

   #201533

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB2
   

   Use

   Synthetic Biology

   Mutation

   rhaR L128+1bp (frameshift), ilvA G455C

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

   Plasmid

   #201535

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB4
   

   Use

   Synthetic Biology

   Mutation

   rhaS ΔM1-H5, PrhaSR Δ179bp

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTW045

   Plasmid

   #185690

   Purpose

   T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

   Depositor

   Feng Zhang

   Article

   Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

   Insert

   Cas9, Drm1b gRNAs
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTW037

   Plasmid

   #185688

   Purpose

   T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

   Depositor

   Feng Zhang

   Article

   Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

   Insert

   Cas9, Drm1b gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149427

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[TraB, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. FG11F-C-FLAG-hNKD2

   Plasmid

   #174330

   Purpose

   encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MO70-C-FLAG-hNKD2

   Plasmid

   #174327

   Purpose

   encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Retroviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKI-cjPLP1e5-S253T

   Plasmid

   #129755

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Mutation

   S253T

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKI-cjPLP1e5-P216S

   Plasmid

   #129754

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Mutation

   P216S

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKI-cjPLP1e2-P15L-_lox

    Plasmid

    #129756

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Mutation

    P15L

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKI-cjPLP1e1-EGFP

    Plasmid

    #129751

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNAs[bTub+Tra].1046D

    Plasmid

    #112693

    Purpose

    express two gRNA targeting bTub & Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA[bTub+DsxF].1046A

    Plasmid

    #112694

    Purpose

    express two gRNA targeting bTub & DsxF under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    K294R

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Gal4-HSF4b WT

    Plasmid

    #118358

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR sgAMDHD1

    Plasmid

    #102316

    Purpose

    genetic depletion of AMDHD1

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgAMDHD1 (AMDHD1 Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30Xa-SBL1(160E)

    Plasmid

    #66846

    Purpose

    Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pet28a_RBPMS_1_120

    Plasmid

    #65737

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-120

    Promoter

    T7

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30Xa-SBL1(160S,NoCys)

    Plasmid

    #66877

    Purpose

    Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    four (all) cysteines mutated to serine

    Promoter

    T7lac

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet28a_RBPMS_1_144

    Plasmid

    #65739

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-144

    Promoter

    T7

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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