We narrowed to 21,768 results for: his

1. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146072

   Purpose

   Insect Expression of DmTral-LSm-R21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21EE23KS13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

   Plasmid

   #146046

   Purpose

   Insect Expression of DmTral-LSm-del46-60

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-del46-60 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

   Plasmid

   #146006

   Purpose

   Insect Expression of DmSmaug-del584-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-del584-762 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   7 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C

   Plasmid

   #146015

   Purpose

   Insect Expression of DmSmaug-del584-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-del584-762 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   7 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

   Plasmid

   #147007

   Purpose

   Insect Expression of DmUPF3-K31E-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3-K31E-siRNAres (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and one non silent mutation P305S compar…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

   Plasmid

   #146990

   Purpose

   Insect Expression of DmUPF1_92-1180-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1_92-1180-siRNAres (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   1 non silent mutations: G995E compared to the seq…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

   Plasmid

   #146901

   Purpose

   Insect Expression of DmCup_1-417-Y342AL347A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

   Insert

   DmCup_1-417-Y342AL347A (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACEBac1-gamma-tubulin(N229A)-TEV-His6

   Plasmid

   #178077

   Purpose

   Insect cell expression of gamma-tubulin(N229A)-TEV-His6

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   gamma-tubulin with an N229A point mutation (TUBG1 Human)
   

   Use

   Tags

   TEV-His6

   Expression

   Insect

   Mutation

   Changed Asparagine 229 to Alanine

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

   Plasmid

   #174426

   Purpose

   Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K569A, K571A

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176577

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

    Plasmid

    #174425

    Purpose

    Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K557A, K560A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-anticalin-FLN-ELP-His-ybbr

    Plasmid

    #168039

    Purpose

    Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid

    #168047

    Purpose

    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCu-His6-Atg13[571–700]-Ss(424)

    Plasmid

    #166852

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE80L-His-SpyTag-XMod(XL2V)-Doc

    Plasmid

    #157676

    Purpose

    Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    XMod-Doc mutant XL2V with Histag and SpyTag
    

    Use

    Tags

    Histag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. T7pr-His6-MBP-TEV-Cas9-BirA*

    Plasmid

    #159993

    Purpose

    Bacterial expression of Cas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    Cas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI-neo-His-MS2BP-G3BP1-S149A

    Plasmid

    #136009

    Purpose

    S149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    MS2BP

    Expression

    Mammalian

    Mutation

    S149A

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IX401: pMVP (L3-L2) 6x His epitope tag + polyA

    Plasmid

    #121754

    Purpose

    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    6x His epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE

    Plasmid

    #121761

    Purpose

    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    6x His epitope tag + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

    Plasmid

    #114621

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

    Plasmid

    #114612

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac Dual PNG172-STII PLU-HIS

    Plasmid

    #112992

    Purpose

    Baculovirus expression of Drosophila png172-Flag and plu-His

    Depositor

    Terry Orr-Weaver

    Inserts

    png172 (png Fly)
    plu (plu Fly)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    png172 mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac Dual PNG-STII PLU-HIS

    Plasmid

    #112993

    Purpose

    Baculovirus expression of Drosophila png-Flag and plu-His

    Depositor

    Terry Orr-Weaver

    Inserts

    png (png Fly)
    plu (plu Fly)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge