We narrowed to 144,958 results for: ins

1. HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

   Plasmid

   #162479

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreBC
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

   Plasmid

   #162474

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreBC
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

   Plasmid

   #162480

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   DNE-Frterminator
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEM271 - MCS mosTI NeoR target

   Plasmid

   #159830

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI NeoR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM254 - sgRNA mosTI neoR

   Plasmid

   #159831

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM322 - array co-injection fragment mosTI neoR

   Plasmid

   #159832

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ414 - DANCER-1

   Plasmid

   #136385

   Purpose

   Expresses DANCER-1 dynamic protein

   Depositor

   Roberto Chica

   Article

   Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

   Insert

   DANCER-1
   

   Use

   Tags

   6x-His tag (N-term of insert)

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

   Promoter

   T7 promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDOC-K-glmS-GFPrev

   Plasmid

   #158060

   Purpose

   Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   Green Fluorescent Protein optimised for excitation with UV light
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   acpP

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQP-2376

   Plasmid

   #138974

   Purpose

   Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB HTB_hMSH6_T1219D

    Plasmid

    #135466

    Purpose

    Expression human MSH6_T1219D mutant protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6_T1219D (MSH6 Human)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    changed threonine to aspartic acid

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

    Plasmid

    #130689

    Purpose

    Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, M1961_E1992del

    Promoter

    CMV

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LC8-like

    Plasmid

    #133115

    Purpose

    Human LC8-like protein, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    LC8-like (LOC392067 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

    Plasmid

    #130690

    Purpose

    Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, G1720_Y1846del

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LI F-G Gent

    Plasmid

    #104522

    Purpose

    LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Apramycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPI467

    Plasmid

    #113208

    Purpose

    integrating expression vector, C-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum integrating expression v…

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPI468

    Plasmid

    #113207

    Purpose

    integrating expression vector, N-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum integrating expression v…

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSO9

    Plasmid

    #106366

    Purpose

    To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.

    Depositor

    Jonathan Ewbank

    Article

    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

    Insert

    ttTi4348_ SEC _pvit-6_OspF_3'unc54
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-R1ACR_6367-EYFP

    Plasmid

    #111080

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin R1ACR_6367
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-RlACR_497-EYFP

    Plasmid

    #111078

    Purpose

    Anion channelrhodopsin from Rhodomonas lens for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RlACR_497
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCh-61

    Plasmid

    #92262

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMCh-59

    Plasmid

    #92261

    Purpose

    Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    aa 940-1215

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS413 Prp5 AAAA

    Plasmid

    #87600

    Purpose

    Encodes Prp5 AAAA for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    PRP5 (PRP5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    D136A, P137A, L138A, D139A

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS413 Prp5 TAG

    Plasmid

    #87603

    Purpose

    Encodes Prp5 SAT motif to TAG for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    PRP5 (PRP5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    S446T, T448G

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGADT7 Hsh155 K335R

    Plasmid

    #87299

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 K335R

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD and HA

    Expression

    Bacterial and Yeast

    Mutation

    K335R

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGBKT7-CLF1

    Plasmid

    #87548

    Purpose

    Encodes Y2H Gal4 DNA binding domain for Clf1

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    CLF1 (CLF1 Budding Yeast)
    

    Use

    Tags

    GAL4 BD and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGADT7 Hsh155 D450G

    Plasmid

    #87303

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 D450G

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD

    Expression

    Bacterial and Yeast

    Mutation

    D450G

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGADT7 Hsh155 P369K

    Plasmid

    #87302

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 P369K

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD

    Expression

    Bacterial and Yeast

    Mutation

    P369K

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGADT7 Hsh155 H331Q

    Plasmid

    #87296

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 H331Q

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD

    Expression

    Bacterial and Yeast

    Mutation

    H331Q

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGADT7 Hsh155 H331D

    Plasmid

    #87295

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 H331D

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD

    Expression

    Bacterial and Yeast

    Mutation

    H331D

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGADT7 Hsh155 N295D

    Plasmid

    #87294

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 N295D

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD

    Expression

    Bacterial and Yeast

    Mutation

    N295D

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge