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We narrowed to 4,853 results for: codon optimized

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Showing: 3221 - 3240 of 4853 results

  3. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

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  4. pETM6-5F5-mCherry

    Plasmid
    #73422
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 5F5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P5F5 (orthogonal T7-lac variant)
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73422-plasmid-map-sequence-id-135322

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  5. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  6. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    209044-plasmid-map-sequence-id-413636

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  7. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized OsCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197024-plasmid-map-sequence-id-388957

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  8. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  9. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Publication
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type (ATP13A2 Human)
    Tags
    BAD tag
    Expression
    Yeast
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171377-plasmid-map-sequence-id-342929

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  10. pCAGGS SARS-CoV-2 BA.2.75 Spike

    Plasmid
    #190012
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike
    Depositor
    Marceline Côté
    Publication
    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:
    Insert
    SARS-CoV-2 BA.2.75 Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the BA.2 mutations (T19I, LPPA24S, G142D…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Sept. 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    190012-plasmid-map-sequence-id-371147

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  13. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    62204-plasmid-map-sequence-id-101817

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  16. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

    Plasmid
    #141372
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    Sept. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    141372-plasmid-map-sequence-id-311193

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  20. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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Showing: 3221 - 3240 of 4853 results