We narrowed to 24,244 results for: crispr

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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sg2.02 16x(MS2) MUC4.1

Plasmid

#101154

Purpose

gRNA with 16 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQAT37

Plasmid

#223409

Purpose

T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Article

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pC017 - LwCas13a guide expression backbone with tRNAval promoter

Plasmid

#91907

Purpose

Backbone for cloning LwCas13a guides under tRNAval promoter

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a Guide site

Expression

Mammalian

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCAS1yl

Plasmid

#73226

Purpose

Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Inserts

Cas9
sgRNA

Use

CRISPR; In y. lipolytica

Expression

Bacterial

Promoter

unknown

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 VPR

Plasmid

#188510

Purpose

Expresses FLAG tagged HiFi dCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAS9id

Plasmid

#141251

Purpose

Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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