Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 3,642 results for: ORF1

Clear keywords
Showing: 3241 - 3260 of 3642 results

  4. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid
    #80640
    Purpose
    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed Glutamate 209 to alanine
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80640-plasmid-map-sequence-id-161707

    Enlarge

  8. pGTM_SUMO_PLpro_C111S

    Plasmid
    #233740
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111S active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    Cys111Ser active site mutation of the Papain-Likeā€¦
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233740-plasmid-map-sequence-id-469323

    Enlarge

  9. pGTM_Sumo2-N-8XHis-Plpro-C111S

    Plasmid
    #234472
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234472-plasmid-map-sequence-id-469247

    Enlarge

  20. pCA528 CHMP4A 153-222

    Plasmid
    #108256
    Purpose
    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4A residues 153-222 (CHMP4A Human)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeliā€¦
    Promoter
    T7
    Available Since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    108256-plasmid-map-sequence-id-245257

    Enlarge

Showing: 3241 - 3260 of 3642 results