We narrowed to 156,021 results for: ins
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Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
TagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
TagssfGFP::2xStrepII tagExpressionInsectPromoterpolyhedrinAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Q443P
Plasmid#216401PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutationDepositorInsertEfg1-ΔDBD-Q443P
Tags6xHis-tagsExpressionBacterialMutationchanged Glutamine 443 to Proline, and deleted ami…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_41BBICD
Plasmid#197097PurposeThis plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of 4-1BB.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_CD28ICD
Plasmid#197098PurposeThis plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of CD28.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
TagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-pmCerulean3-N1
Plasmid#204958PurposeExpression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cellsDepositorInsertMsACR1
TagsmCerulean3ExpressionMammalianPromoterCMV (+enhancer)Available SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW2-MaUPPS-B
Plasmid#203175PurposeYeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertMA_4402
ExpressionYeastPromoterTDH3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-MaUPPS-A
Plasmid#203174PurposeYeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertuppS
ExpressionYeastPromoterTDH3Available SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CePAN3_1-244_P
Plasmid#147243PurposeInsect Expression of CePAN3_1-244DepositorInsertCePAN3_1-244
ExpressionInsectMutationInsertion of two AA (FQ) at position 172 compared…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAL1-VP1 (LEU2)
Plasmid#182480PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertdeltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM262 - pDESTR4-R3 mosTI hygroR target
Plasmid#159840PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpDESTR4-R3 mosTI hygroR target
ExpressionWormMutationNot applicableAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6A)
Plasmid#171552PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa six-alanine insertion in the L1 regionAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
ExpressionBacterial and YeastAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP9
Plasmid#175793Purposede novo thick circular tandem repeat proteins with 9 repeatsDepositorInserttcTRP9
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+3A)
Plasmid#171551PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa three-alanine insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6)
Plasmid#171550PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (L)
Plasmid#170624PurposeFor the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539EDepositorInsertLyn11-myc-LOV2GIVe (L)
TagsmycExpressionMammalianPromoterCMVAvailable SinceJuly 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC-lyn11-myc-LOV2GIVe (D)
Plasmid#170625PurposeFor the mammalian expression of mLOV2GIVe dark-mimicking mutation C450SDepositorInsertLyn11-myc-LOV2GIVe (D)
TagsmycExpressionMammalianPromoterCMVAvailable SinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
KG#471
Plasmid#110935PurposeExpresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tailDepositorInsertsmig-13 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20F
Plasmid#145789PurposemPing Yeast Transposition AssayDepositorInsertmmPing20F
ExpressionYeastMutationInsertion of the FMV enhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20B
Plasmid#145790PurposemPing Yeast Transposition AssayDepositorInsertmmPing20B
ExpressionYeastMutationInsertion of the b-Conglycin EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A NL60
Plasmid#145791PurposemPing Yeast Transposition AssayDepositorInsertNL60
ExpressionYeastMutationInsertion of the OCS EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 2xE
Plasmid#145792PurposemPing Yeast Transposition AssayDepositorInsert2xE
ExpressionYeastMutationInsertion of 2 copies of the CMV EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 4xE
Plasmid#145793PurposemPing Yeast Transposition AssayDepositorInsert4XE
ExpressionYeastMutationInsertion of 4 copies of the CMV EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw
Plasmid#162479PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreBC
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw
Plasmid#162474PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreBC
UseCre/LoxAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only