We narrowed to 10,003 results for: SEC

1. pAAV-pEF1a-EBFP-tTAn

   Plasmid

   #172122

   Purpose

   Constitutive expression of EBFP and rtTA-InteinN

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDR111_Bgal no NLS

   Plasmid

   #188397

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Phyper spank

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti EF1a GFP-mCherry-mPlin1

   Plasmid

   #189005

   Purpose

   Fluorescent reportor for lipophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   GFP, mChery, Plin1 (Plin1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CIB81-pmGFP

   Plasmid

   #75366

   Purpose

   Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   prenylated EGFP

   Expression

   Mammalian

   Mutation

   residues 1-81 of CIB1

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Arf Q71L CFP

   Plasmid

   #128149

   Purpose

   Expression of Arf Q71L

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 2002 May 9;417(6885):187-93. doi

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q71L

   Promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 GATA1

   Plasmid

   #85693

   Purpose

   Mammalian expression of mouse GATA1

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

   Insert

   GATA1 (Gata1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACEBac1-PARP2-Flag-His-WT

   Plasmid

   #111574

   Purpose

   Expresses full length PARP2 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP2 (PARP2 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SAE1

   Plasmid

   #175241

   Purpose

   Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SAE1 (SAE1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SAE2-mGarnet

    Plasmid

    #175240

    Purpose

    Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mH2A1.1-CT-GFP

    Plasmid

    #45167

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GW1-rogRFP2

    Plasmid

    #207876

    Purpose

    Mammalian expression of the red fluorescent rogRFP2 redox sensor

    Depositor

    Mathew Tantama

    Article

    Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

    Insert

    rogRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Str-KDEL_ManII-SBP-mCherry

    Plasmid

    #65253

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeonGreen-α-synuclein

    Plasmid

    #205713

    Purpose

    Mammalian expression of mNeonGreen-alpha-synuclein

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFast-hHus1

    Plasmid

    #222469

    Purpose

    Expresses hHus1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hHus1 (HUS1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFast-Flag-hHus1

    Plasmid

    #222470

    Purpose

    Expresses hHus1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hHus1 (HUS1 Human)
    

    Use

    Tags

    Flag epitope

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TNF-SBP-EGFP_puromycin

    Plasmid

    #65284

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-KDEL_ST-SBP-mCherry

    Plasmid

    #65265

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-IRES-tdTomato-FlagAkt1

    Plasmid

    #64831

    Purpose

    Lentiviral expression of murine Flag-Akt1

    Depositor

    Eva Gonzalez

    Article

    Kajno et al Biochem J. 2015 Apr 9.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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