Skip to main content
Addgene
Showing: 1 - 20 of 10138 results

  1. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. IQSEC2 (IQSEC2A-c001)

    Plasmid
    #110259
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    IQSEC2 (IQSEC2 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110259-plasmid-map-sequence-id-214719

    Enlarge

  4. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

    Enlarge

  8. SEC14L3

    Plasmid
    #73240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L3 (SEC14L3 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. SEC14L4

    Plasmid
    #73233
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEC14L4 (SEC14L4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73233-plasmid-map-sequence-id-137211

    Enlarge

  10. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

    Enlarge

  14. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

    Enlarge

  15. pSecEffector

    Plasmid
    #172891
    Purpose
    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
    Depositor
    Urartu Seker
    Article
    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696
    Inserts
    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ProD, nTETO, and pBAD /AraC
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172891-plasmid-map-sequence-id-368224

    Enlarge

  16. secCitrine

    Plasmid
    #181964
    Purpose
    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter
    Depositor
    George Haughn
    Article
    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do
    Insert
    signal peptide sequence from basic chitinase
    Use
    Tags
    Citrine YFP
    Expression
    Plant
    Mutation
    Promoter
    1.5 kb MUM4 promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    181964-plasmid-map-sequence-id-356728

    Enlarge

  19. psecBFP2

    Plasmid
    #62230
    Purpose
    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
    Depositor
    Erik Snapp
    Article
    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;
    Insert
    secBFP2
    Use
    Tags
    secbfp2
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. mGoosecoid

    Plasmid
    #25777
    Depositor
    Edward De Robertis
    Insert
    Goosecoid (Gsc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 10138 results