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pDONR223-MAP3K8

Plasmid

#23538

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIND21-puro_SOX10-OLIG2-NKX6.2-3xFLAG

Plasmid

#250320

Purpose

Tetracycline-inducible expression of SOX10-OLIG2-NKX6.2 as polyprotein linked by self cleavage sites P2A and T2A. rtTA (Tet-ON) is driven the EF1alpha promoter.

Depositor

Michael Wehr

Article

Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33

Insert

SOX10-P2A-OLIG2-T2A-NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

Plasmid

#164450

Purpose

AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Has Service

AAV PHP.eB

Insert

iCre

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)

Plasmid

#224052

Purpose

AiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1848 - pAAV-AiE2435m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214552

Purpose

AiE2435m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1911 - pAAV-AiE2114m-minBG-iCre-WPRE-BGHpA

Plasmid

#220719

Purpose

AiE2114m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11969 - pAAV-hsA2-AiE0140h-minRho-SYFP2-4X2C-WPRE-HGHpA (Alias: CN1969)

Plasmid

#223969

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15262 - pAAV-AiE0219h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5262)

Plasmid

#224062

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14211 - pAAV-DLX2.0-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4211)

Plasmid

#229936

Purpose

DLX2.0 is an enhancer sequence, designed to drive nuclear mScarlet expression in forebrain GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mScarlet

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14019 - pAAV-hSyn1-mTFP1-10aa-H2B-WPRE3-BGHpA (Alias: CN4019)

Plasmid

#229937

Purpose

AAV vector for pan-neuronal expression of nuclear mTFP1

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mTFP1-10aa-H2B

Use

AAV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12309 - pAAV-AiE0130h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2309)

Plasmid

#230199

Purpose

AiE0130h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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