We narrowed to 2,441 results for: organization Addgene

1. ZJOM2

   Plasmid

   #133633

   Purpose

   Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VRG4-GFP (VRG4 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PhyB-mCherry-Htb2

   Plasmid

   #51569

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   HTB2 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM141

   Plasmid

   #133669

   Purpose

   Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PhyB-mCherry-PEX3

   Plasmid

   #51572

   Purpose

   Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   PEX3 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

   Plasmid

   #127360

   Purpose

   gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   R118G

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLaNGo11-1

   Plasmid

   #126053

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TPC2-D276K-G-GECO1.2

   Plasmid

   #207142

   Purpose

   Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Changed Aspartate 276 to Lysine

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM41-EcLonA

   Plasmid

   #38330

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   Lon protease fragment (lon Escherichia coli)
   

   Use

   Tags

   His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   Polyphosphate binding fragment of Lon protease. …

   Promoter

   T7

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

   Plasmid

   #127357

   Purpose

   gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   R118G

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-HT_ygiF-CHAD

    Plasmid

    #127793

    Purpose

    Expresses the CHAD domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF-CHAD (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 201-433)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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