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pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Use

Tags

mEos3.2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Article

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pltetO1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL7026

Plasmid

#128811

Purpose

Fluoride riboswitch-regulated 3-way junction dimeric broccoli

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated 3-way junction dimeric broccoli

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0531

Plasmid

#128988

Purpose

Part plasmid for VP16-CIB1, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-VP16-CIB1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB401NL3F10H

Plasmid

#141285

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#24636

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-V220A

Plasmid

#47645

Purpose

pAC-EsaR with a c659t mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-V220A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Valine 220 to Alanine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
CDS_aadA_chlo

Plasmid

#163935

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

aadA gene for spectinomycin resistance

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST1K18ms

Plasmid

#72646

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Tags

Expression

Mutation

Promoter

Available since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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AEB-F12

Plasmid

#63849

Purpose

This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

F12 fluoride riboswitch

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23100

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBbA1c-RFP

Plasmid

#35335

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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