We narrowed to 4,952 results for: mcs

1. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

   Plasmid

   #175816

   Purpose

   pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags

   Depositor

   Mark Banfield, Lennart Wirthmueller

   Article

   Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

   Insert

   IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Mutation

   natural polymorphism P65S, annotated in Genbank f…

   Promoter

   none

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

   Plasmid

   #169468

   Purpose

   Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

   Plasmid

   #169466

   Purpose

   Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgZmat3.51.5/Cre

   Plasmid

   #167855

   Purpose

   Expresses Cre recombinase and an sgRNA targeting Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   gRNA targeting Zmat3 (Zmat3 Mouse)
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSico_U6-PGC1a1 sgRNA

   Plasmid

   #165425

   Purpose

   Expression of gRNA against human PGC-1a variant 1

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   gRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

6. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

   Plasmid

   #156417

   Purpose

   8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
   

   Use

   Zebrafish transgenesis

   Promoter

   E1b min pro/b-globin intron

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-rPKL S12TAG

   Plasmid

   #107155

   Purpose

   MBP-rPKL construct for production and purification of rat PKL pS12

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 12 to TAG for phosphoserine insertion

   Promoter

   PBAD

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-rPKL S113TAG

   Plasmid

   #107157

   Purpose

   MBP-rPKL S113TAG for production and purification of rat PKL pS113

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 113 changed to TAG for phosphoserine inser…

   Promoter

   PBAD

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-RSLID1(WT)

   Plasmid

   #122296

   Purpose

   expresses RSL1D1 protein in mammalian cells (puro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   vector's LTR

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIR-31-192-193a-Reporter

    Plasmid

    #71874

    Purpose

    Mammalian expression vector for the analysis of miR-31-19α-193a activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Binding sequence targeted by miR-31, miR192, and miR-193a-5p
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73040

    Purpose

    Express Nluc and copGFP dual reporter in mammalian cells

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    PGK (phosphoglycerate kinase 1)

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-mitfa2.1

    Plasmid

    #81234

    Purpose

    zebrafish mitfa promoter entry vector for multisite Gateway cloning

    Depositor

    James Lister

    Insert

    mitfa promoter (mitfa Zebrafish)
    

    Use

    Multisite gateway entry vector

    Promoter

    mitfa, 2.1kb including 5' UTR

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2MP.EF-QUE37C

    Plasmid

    #129332

    Purpose

    ATP measurement, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Expression

    Mammalian

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHM830

    Plasmid

    #20702

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-MCS-LacZ
    

    Expression

    Mammalian

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV asyn S129A

    Plasmid

    #36066

    Depositor

    Hilal Lashuel, Patrick Aebischer

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S129A

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid

    #72255

    Purpose

    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter

    Depositor

    Kazuhiro Oka

    Insert

    iCre-P2A-tdTomato-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available Since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73039

    Purpose

    Express Nluc, copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    chicken beta-actin + CMV enhancer and chicken bet…

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV asyn S129D

    Plasmid

    #36069

    Depositor

    Hilal Lashuel, Patrick Aebischer

    Insert

    alpha-synuclein S129D (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S129D

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pT2MP.EF-pHlu

    Plasmid

    #129304

    Purpose

    pH measurement, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Expression

    Mammalian

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2MPEF-TOMM-QUE37C

    Plasmid

    #191526

    Purpose

    ATP measurement, mitochondria outer membrane, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Expression

    Mammalian

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge