We narrowed to 15,088 results for: pET

1. pET28A-p53 (1-73) (P12A P13A)

   Plasmid

   #62066

   Purpose

   Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A

   Promoter

   T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET-CH2 (1192-1318)

   Plasmid

   #99338

   Purpose

   Bacterial expression of mouse CBP CH2 domain

   Depositor

   Peter Wright

   Article

   Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

   Insert

   CBP CH2 (1192-1318) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET11a-DAAT-V33G/T242G

   Plasmid

   #159278

   Purpose

   Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

   Depositor

   Roberto Chica

   Article

   Parmeggiani et al ACS Catalysis 9, 3482–3486.

   Insert

   Engineered d-alanine aminotransferase
   

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Valine 33 and threonine 242 have been mutated to …

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a-mAIM2-deltaPYD-GST

   Plasmid

   #51534

   Purpose

   Express GST-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   GST and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM6-At4CL-PhCHS-MsCHI

   Plasmid

   #73392

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM6-Vv4CL-CmCHS-MsCHI

   Plasmid

   #73402

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Vv4CL
   CmCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETDuet RagA-RagC(L91P)

   Plasmid

   #99647

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L91P

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HOXA9 (murine) FLAG pET

   Plasmid

   #8494

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA9 (Hoxa9 Mouse)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   FLAG epitope tag replaces T7 tag in the pET back…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET151-Vta1(280-330)

   Plasmid

   #87738

   Purpose

   Bacterial expression of the VSL domain of S. cerevisiae Vta1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-279

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-28a(+)-MVK jannaschii

    Plasmid

    #108948

    Purpose

    heterologous expression of MVK of M. jannaschii in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid

    #138208

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub(T/A)-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available Since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

    Plasmid

    #117909

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R98A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    Strep-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pET21a-hAIM2-PYD-GST

    Plasmid

    #51531

    Purpose

    Express GST-tagged PYD of hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Tags

    GST and T7

    Expression

    Bacterial

    Mutation

    deletion of amino acids 102-343

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge