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DTYMK gRNA (BRDN0001145924)

Plasmid

#77513

Purpose

3rd generation lentiviral gRNA plasmid targeting human DTYMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DTYMK (DTYMK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK10 gRNA (BRDN0001145808)

Plasmid

#77578

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK10 (CDK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK10 gRNA (BRDN0001149446)

Plasmid

#77579

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK10 (CDK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001144753)

Plasmid

#76760

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001147643)

Plasmid

#76761

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001147398)

Plasmid

#76762

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001145152)

Plasmid

#75579

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001146504)

Plasmid

#75580

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001147779)

Plasmid

#75581

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DTYMK gRNA (BRDN0001147404)

Plasmid

#77511

Purpose

3rd generation lentiviral gRNA plasmid targeting human DTYMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DTYMK (DTYMK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DTYMK gRNA (BRDN0001148632)

Plasmid

#77512

Purpose

3rd generation lentiviral gRNA plasmid targeting human DTYMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DTYMK (DTYMK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001145453)

Plasmid

#75640

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001146985)

Plasmid

#75641

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001147486)

Plasmid

#75642

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
NC12 pCDNA3.1 VENUS WTX delta ETGE

Plasmid

#36967

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX (delta ETGE motif) (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

lacks amino acids 288 –291

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
AAV Syn-FLEX-coca-5HT3-IRES-mCherry

Plasmid

#242195

Purpose

Cre-dependent AAV vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-FLEX-coca-5HT3-IRES-mCherry (Htr3a Mouse)

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-HP1a-gRNA-2

Plasmid

#237635

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HP1a gRNA (CBX5 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1-KS

Plasmid

#237679

Purpose

For overexpression of mEGFP-SS18-SSX1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1-KS (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1

Plasmid

#237678

Purpose

For overexpression of mEGFP-SS18-SSX1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1 (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2-KS

Plasmid

#237680

Purpose

For overexpression of mEGFP-SS18-SSX2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2-KS (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-HP1a-gRNA-1

Plasmid

#237634

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HP1a gRNA (CBX5 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-TCOF1-gRNA

Plasmid

#237633

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 gRNA (TCOF1 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2037

Plasmid

#144378

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP53BP1 (TP53BP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLX313_PELO_WT

Plasmid

#228938

Purpose

Constitutive expression of wild-type human PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD051d_sgCiPELO #2

Plasmid

#229018

Purpose

Expression of CRISPRi PELO sgRNA #2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD051d_sgCiPELO #1

Plasmid

#229017

Purpose

Expression of CRISPRi PELO sgRNA #1

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K4-10A

Plasmid

#229403

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K4 (MAP4K4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S523,S536,S547,S643,T684,S726…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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