We narrowed to 3,483 results for: mpo
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Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
TagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)Available SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-hCas3
Plasmid#134920PurposeComponents for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.DepositorInsertCas3 with bpNLS
ExpressionMammalianPromoterCAGAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM1110_LEU2
Plasmid#168460PurposeBig-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.DepositorInsertmRFP1
UseSynthetic Biology; Yeast assembly vector for big-…Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVA1
Plasmid#58953PurposeContains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.DepositorInsertVAR1u, VAR1 recoded for expression from the nucleus
UseYeast ecoli shuttle vectorTagspreCox4 mitochondrial matrix targeting signalExpressionYeastMutationSynthetic gene in standard genetic codePromoterADH1Available SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF
Plasmid#225828PurposeA component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)DepositorInsertmVenus-FKBP-K7GEF
UseAAVExpressionMammalianAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 BbsI
Plasmid#155184PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backboneDepositorTypeEmpty backboneUseUnspecified; Piggybac transposonExpressionMammalianPromoterU6Available SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-YFP
Plasmid#1816PurposeMammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
TagsYFPExpressionMammalianMutationD50E (not important for function of plasmid)Available SinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterAtUBI10Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1
Plasmid#101764PurposeExpresses PSPC1 in mammalian cellsDepositorAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5
Plasmid#81062PurposeGateway entry vector encoding full length UBR5DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
UseCloningMutationK503RPromoterN/AAvailable SinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LiPOP2
Plasmid#168266PurposeA single-component optogenetic tool for photoswitchable pyroptosisDepositorInsertmGSDMD(1-276) (Gsdmd Mouse)
TagscpLOV2, mCherryExpressionMammalianMutationR138A, K146A, R152A, R154APromoterCMVAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-3XFLAG-AGO2
Plasmid#136687PurposeExpresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)DepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBS-AMVpl-β1WT-nAchR
Plasmid#97080PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertBeta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB405-TIC21-sGFP
Plasmid#213865PurposeBinary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .DepositorInsertchloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS
Plasmid#62861PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEA1
Plasmid#176527PurposeDelivers all prime editing nickase components in a single plasmidDepositorInsertCbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseCRISPRExpressionMammalianPromoterCbH for Cas9, hU6 for gRNAsAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(CmYLCV)
Plasmid#202014PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterCmYLCVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only