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p3xFLAG-CMV-9-sGPR124 ΔGAIN-His

Plasmid

#246693

Purpose

Expression vector for secreted human GPR124 ECD lacking the GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 427-755

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔHRM-His

Plasmid

#246692

Purpose

Expression vector for secreted human GPR124 ECD lacking the HRM domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 349-426

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-GPR124 GAIN-His

Plasmid

#246694

Purpose

Expression vector for secreted human GPR124 GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

GAIN domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-GPR124 HRM-GAIN-His

Plasmid

#246696

Purpose

Expression vector for secreted human GPR124 HRM and GAIN domains, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

HRM-GAIN fragment

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124-His

Plasmid

#246685

Purpose

Expression vector for secreted human GPR124 ECD, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124 ΔLRR-His

Plasmid

#246691

Purpose

Expression vector for secreted human GPR124 ECD lacking LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 34-247

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MPC1-QQ-FLAG

Plasmid

#228905

Purpose

Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tag

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K45 and K46 motif sites were both replaced with g…

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-W

Plasmid

#200487

Purpose

Lentiviral vector expressing gRNA targeting human CDK6-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6-E1(6) (CDK6 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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