We narrowed to 8,239 results for: FIE

1. pET:GCAT:GFP

   Plasmid

   #60736

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiGuide_mCherry_sgEPAS1#2

   Plasmid

   #183123

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET:ACAT:GFP

   Plasmid

   #60740

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFTK086

   Plasmid

   #171358

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HL 818 (=HL 716 + pHL 98)

   Plasmid

   #30006

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Insert

   pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp
   

   Use

   Synthetic Biology

   Tags

   mcherry, GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pSUPER-retro-puro-shNup35-EcoRI

   Plasmid

   #87332

   Purpose

   To express shRNA against human Nup35

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup35
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-STOP-shA1

   Plasmid

   #14422

   Depositor

   Klaus Rajewsky

   Article

   Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

   Insert

   modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
   

   Use

   Cre/Lox and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSW179 - pML1

   Plasmid

   #115977

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Jan Lohmann

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pML1
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXW109PmerT-Pecf11-gfp

   Plasmid

   #160822

   Purpose

   Two-layered mercury responsive circuit carrying an ECF11-based amplifier

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

   Insert

   PJ23109-32merR-t-PmerT-33ecf11-Pecf11-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23109, PmerT, Pecf11

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Syn3D33A

    Plasmid

    #13400

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. D33 of s…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Syn3E34A

    Plasmid

    #13401

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. E34 of s…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pET:CCCT:GFP

    Plasmid

    #60738

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nPEX-LV

    Plasmid

    #12119

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    noncatalytic fragment of MMP2 amplified via PCR, …

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pUAP1_Ta_Yr7_CDS_no_STOP

    Plasmid

    #141093

    Purpose

    Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

    Depositor

    Cristobal Uauy

    Article

    Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

    Insert

    Ta_Yr7
    

    Use

    Unspecified; Level 0 module for golden gate cloni…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-CMV-p85a 3'UTR

    Plasmid

    #51392

    Depositor

    Narry Kim

    Article

    Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

    Insert

    p85alpha 3'UTR (PIK3R1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-Chronos(M140T)-mRuby2-ST

    Plasmid

    #109129

    Purpose

    Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    Chronos(M140T)-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HL 3904 (=HL 770 + pHL 991)

    Plasmid

    #30093

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMOD_C4300

    Plasmid

    #91103

    Purpose

    Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BaeI LacZ cassette for GT donor cloning
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JH05

    Plasmid

    #73516

    Purpose

    mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mPYet::PH::tbb-2
    

    Use

    Tags

    mYPet

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-TGF beta type I receptor (mL45TD)

    Plasmid

    #34702

    Depositor

    Ying Zhang

    Article

    Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T202D, N265A, D267A, N268A

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

21. Cdc42 T17N pCS2

    Plasmid

    #16670

    Depositor

    De-Li Shi

    Article

    Djiane et al Development. 2000 Jul . 127(14):3091-100

    Insert

    Cdc42 T17N (CDC42 Human)
    

    Use

    Vertebrate

    Tags

    Expression

    Mutation

    T17N. Dominant negative.

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

22. Syn3F31A

    Plasmid

    #13398

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. F31 of s…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

23. 36INS-J23100_GB

    Plasmid

    #78669

    Purpose

    36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 36INS-J23100_EB

    Plasmid

    #78667

    Purpose

    36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBabe-N-ZAP-Zeo

    Plasmid

    #17382

    Depositor

    Stephen Goff

    Article

    Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

    Insert

    NZAP (Zc3hav1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal region of ZAP (amino acids 1-254)

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEM4Z globin NB Probe

    Plasmid

    #120390

    Purpose

    Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

    Depositor

    Niels Gehring

    Article

    Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

    Insert

    beta globin partial 3' UTR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HL 3722 (=HL 716 + pHL 404)

    Plasmid

    #30084

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

28. Cerulean(159-238) in pcDNAI/Amp

    Plasmid

    #60898

    Purpose

    This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    Cerulean(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains N164H substitution

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pClneoFLAG mouse RASSF2

    Plasmid

    #37017

    Depositor

    Yutaka Hata

    Article

    Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

    Insert

    RASSF2 (Rassf2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D72G and S127F

    Promoter

    CMV

    Available since

    Aug. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pSUPER-retro-puro-shNup93-SpeI

    Plasmid

    #87331

    Purpose

    Expression of shRNA targeting at Nup93

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup93
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFP140

    Plasmid

    #21672

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

32. pFP120

    Plasmid

    #21673

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, a 918…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pSUPER-retro-puro-shNup88-HindIII

    Plasmid

    #87329

    Purpose

    To express shRNA against human Nup88

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup88
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Desmin/pBSSK+

    Plasmid

    #97207

    Purpose

    Desmin (NM_001927, nt 258 to 395) in pBSSK+

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    Desmin (DES Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. HL 4175 (=HL 716 + pHL 1391)

    Plasmid

    #30096

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLlacO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

36. HL 1329 (=HL 865 + pHL 344)

    Plasmid

    #30014

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:mCherry, pLlacO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    mCherry, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

37. HL 2818 (=HL 865 + pHL 489)

    Plasmid

    #30032

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

38. Syn3D146

    Plasmid

    #14677

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-146…

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

39. Syn3-27-146+TM

    Plasmid

    #14679

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 27-14…

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

40. HL 4218 (=HL 716 + pHL 1411)

    Plasmid

    #30105

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Insert

    pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp
    

    Use

    Synthetic Biology

    Tags

    T7noRBS, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only