We narrowed to 23,448 results for: Spr;

1. B52 (empty plasmid backbone to express 2 sgRNAs)

   Plasmid

   #100708

   Purpose

   Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. VCP F12.4 gRNA

   Plasmid

   #90939

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human VCP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   VCP (Guide Designation F12.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VCP F11.4 gRNA

   Plasmid

   #90938

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human VCP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   VCP (Guide Designation F11.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-CDDR

   Plasmid

   #149355

   Purpose

   CRISPR/Cas9-based dual-fluorescent DSB repair reporter

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   CDDR Reporter
   Blasticidin resistance
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hPGK

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-AsCpf1-Blast

   Plasmid

   #84750

   Purpose

   Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   AsCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas9-EcRT-GAL-HIS3

   Plasmid

   #232093

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas9-EcRT-GAL-Nat

   Plasmid

   #232090

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas9-EcRT-GAL-Kan

   Plasmid

   #232092

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BRCA2 A12.2 gRNA

    Plasmid

    #90551

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcU6_1 sgRNA

    Plasmid

    #92395

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.1 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.1

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCTRE-CD4

    Plasmid

    #114010

    Purpose

    Dox-inducible Cas9 expression with a CD4 selection marker

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-ABE7.10-P2A-GFP

    Plasmid

    #162509

    Purpose

    Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

    Insert

    ABE7.10-P2A-GFP
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

    Plasmid

    #233078

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OA-986F (AAEL003877-dCas9-VPR)

    Plasmid

    #183993

    Purpose

    Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    AAEL003877-dCAS9-VPR
    

    Use

    CRISPR

    Tags

    dCas9-VPR

    Expression

    Insect

    Mutation

    Promoter

    AAEL003887

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge