We narrowed to 24,438 results for: nes

1. pMaCTag-33

   Plasmid

   #119986

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry pBSDONR P4r-P2

   Plasmid

   #121528

   Purpose

   For making C-terminal mCherry fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Article

   Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330A_dCas9-1x5

   Plasmid

   #63599

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST HP1 gamma deltaC

   Plasmid

   #24077

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 gamma (CBX3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Missing the C-terminal 25 amino acids

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pX330A_dCas9-1x3

   Plasmid

   #63597

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. KIF1C-FHA

   Plasmid

   #25549

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   KIF1C-FHA (KIF1C Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   FHA domain only

   Promoter

   Available since

   Aug. 25, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pFLIP30

   Plasmid

   #65499

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-AFPt9-attR1 and attR2-mCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLIP32

   Plasmid

   #65500

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLIP39

   Plasmid

   #65507

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSLZ.D

    Plasmid

    #51496

    Purpose

    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. U371-GFP ILtoEK

    Plasmid

    #24472

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (unc-104 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Contains only the kinesin's motor head fused…

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pccGFP-MalE-pos-2H01

    Plasmid

    #239760

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of SEM5A
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Leucine 985 to Isoleucine

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pccGFP-MalE-neg-2H11

    Plasmid

    #239086

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of EVA1B
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Leucine 30 to Isoleucine

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pccGFP-MalE-pos-1F02

    Plasmid

    #239087

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of AMPE
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    deleted amino acids 19-22, and changed Leucine 35…

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pccGFP-MalE-pos-2H07

    Plasmid

    #239088

    Purpose

    Positive control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of FND10
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 184 to Isoleucine

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pccGFP-MalE-neg-2E11

    Plasmid

    #239084

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of LRIG3
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 827 to Isoleucine

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pccGFP-MalE-neg-2F12

    Plasmid

    #239085

    Purpose

    Negative control for liquid MalE complementation assay

    Depositor

    Alessandro Senes

    Article

    Anderson et al bioRxiv 2025.04.22.650048

    Insert

    transmembrane domain of LRIG3
    

    Use

    Tags

    MBP and ToxR

    Expression

    Bacterial

    Mutation

    Changed Valine 815 to Isoleucine

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJPC055

    Plasmid

    #234536

    Purpose

    TRV2 expressing Cre

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    Cre recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-luxNeon

    Plasmid

    #229942

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxNeon
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. JD73

    Plasmid

    #229482

    Purpose

    Adenoviral VA RNA I & II genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    VA RNA I & II
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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