We narrowed to 3,806 results for: golden gate

1. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL475

   Plasmid

   #219503

   Purpose

   Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gly-tRNA-gRNA scaffold for library 2

   Plasmid

   #192365

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pro-tRNA-gRNA scaffold for library 2

   Plasmid

   #192366

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Pro tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gly-tRNA-gRNA scaffold for library 1

   Plasmid

   #192368

   Purpose

   tRNA sequence provider (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pro-tRNA-gRNA scaffold for library 1

   Plasmid

   #192369

   Purpose

   tRNA sequence provider (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Pro tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPQD0KN0751

   Plasmid

   #185628

   Purpose

   Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   lacZ
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mt-DdCBE G1397-C

   Plasmid

   #174563

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1397-C
   

   Tags

   FLAG

   Expression

   Plant

   Promoter

   PcUbi

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mt-DdCBE G1397-N

   Plasmid

   #174562

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1397-N
   

   Tags

   HA

   Expression

   Plant

   Promoter

   PcUbi

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. cp-DdCBE G1397-C

    Plasmid

    #174559

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-C
    

    Tags

    FLAG

    Expression

    Plant

    Promoter

    PcUbi

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. cp-DdCBE G1397-N

    Plasmid

    #174558

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1397-N
    

    Tags

    HA

    Expression

    Plant

    Promoter

    PcUbi

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC71102

    Plasmid

    #154065

    Purpose

    Level 0 Golden Gate vector; Unmodifed CRE

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    CRE
    

    Use

    Synthetic Biology; Standard cloning vector used b…

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL11062

    Plasmid

    #68256

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_AtU6-26 + sgRNA_BolC.GA4a-2
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK435

    Plasmid

    #236573

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDC32B-NbmiR482aTS-B/c

    Plasmid

    #227967

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Expression

    Plant

    Promoter

    35S and None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNP3254 [pNP2922 - ISCro4 Enhanced DBL (DBL3, WT) + ISCro4 Recombianse (WT]

    Plasmid

    #247257

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA101-179 ins179(rc(101-111) (DBL3)
    

    Expression

    Mammalian

    Mutation

    ins179(rc(101-111)

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247261

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL4)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid

    #192453

    Purpose

    intronized ttLbCas12a for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Mutation

    temperature-tolerant mutation (D156R)

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBG-ttLbUV2

    Plasmid

    #231386

    Purpose

    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    ttLbCas12a Ultra V2
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D156R and E795L

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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