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pcDNA3.1-HaloEKAR2.2-T2A-EGFP

Plasmid

#216495

Purpose

Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloEKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hHus1

Plasmid

#222470

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Tags

Flag epitope

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ40-Flag-iBox-PAK4cat

Plasmid

#190178

Purpose

expresses Flag-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

iBox-PAK4cat

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/mScarlet(i)-hCdt100Nt

Plasmid

#129334

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet(i) (Addgene 85044)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Expression

Bacterial

Promoter

Pc

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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CeNL-Actin_pcDNA3

Plasmid

#89512

Purpose

Cyan color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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YeNL-H2B_pcDNA3

Plasmid

#89522

Purpose

Yellow color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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CTA

Plasmid

#27804

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pREC1010C-Eco1

Plasmid

#240690

Purpose

RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-SspB-PPM1H

Plasmid

#232870

Purpose

Expresses SspB-PPM1H in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

Halo-SspB-PPM1H (PPM1H )

Tags

Halo-SspB

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

Plasmid

#231597

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_iRFP670

Plasmid

#191749

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and iRFP670

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_vsfGFP9

Plasmid

#191747

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and vsfGFP-9

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-Tight2-AkaLuc

Plasmid

#186186

Purpose

AAV vector packaging AkaLuciferase under the TRE promoter

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

AkaLuc luciferase

Use

AAV

Promoter

TRE-Tight2

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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