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pGL1_36

Plasmid

#199043

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI plus NGS barcode 13

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_21 [UARS1 ORI]

Plasmid

#198934

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

UARS1 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pRSETB

Plasmid

#55775

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

6xHis and msEGFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pAJ08

Plasmid

#14912

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis actin promoter

Tags

GFP

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET2

Plasmid

#107435

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET2 (MET2 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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LEXY TR (with KRAB domain) (pDN163)

Plasmid

#72662

Purpose

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET3

Plasmid

#107436

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET3 (MET3 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-MCS1M(8)Kana-mCMV-pA

Plasmid

#22059

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-pA expression cassette

Expression

Mammalian

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only
pYES2.1-cerMET14

Plasmid

#107441

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET14 (MET14 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPtac

Plasmid

#166937

Purpose

Ptac single-level gene expression controller (SLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- PTAC

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.SP146.gp91.GP91.cHS4

Plasmid

#30480

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Antisense

Plasmid

#60632

Purpose

Negative Control for the pcDNA3.1(+) ZKSCAN1 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-let-7-A114-N40

Plasmid

#50563

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-let7-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Rad S105N

Plasmid

#11214

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Mutation

S105N

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pLV.CMVenh.gp91.GP91.cHS4

Plasmid

#30479

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0029

Plasmid

#162311

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0284

Plasmid

#162289

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pACSA_Pcpt_YFP

Plasmid

#82019

Purpose

pALM34; control for YFP expression from cyanobacterial phycocyanin truncated promoter

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Promoter

Pcpt

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0028

Plasmid

#162310

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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