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NK639

Plasmid

#109102

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIAGO-AceF1

Plasmid

#114461

Purpose

Expression of late polyene biosynthetic genes in streptomycetes

Depositor

Patrick Caffrey

Article

Stephens et al Biosci Biotechnol Biochem. 2013;77(4):88

Insert

AceDI + AceDII + AceN + AceM

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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NK640

Plasmid

#109103

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK642

Plasmid

#109101

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK586

Plasmid

#109069

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_05705

Use

Choanoflagellate expression

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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NK613

Plasmid

#109091

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry_SV40 NLS

Use

Choanoflagellate expression

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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NK648

Plasmid

#109104

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

3'UTR EFL

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK597

Plasmid

#109086

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

tdTomato

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK595

Plasmid

#109083

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

EGFP

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK585

Plasmid

#109079

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK609

Plasmid

#109078

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Mutation

Eliminate NcoI site in the promoter

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK583

Plasmid

#109072

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_10057

Use

Choanoflagellate expression

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK600

Plasmid

#109068

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_02256

Use

Choanoflagellate expression

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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BTV

Plasmid

#84771

Purpose

bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production

Depositor

David Erle

Article

Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CA-NFAT2

Plasmid

#11102

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Mutation

Constitutively active: multiple serine to alanine…

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Expression

Yeast

Promoter

ENO2 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Expression

Yeast

Promoter

SSA1 S.c.

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2

Plasmid

#113634

Purpose

An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

Depositor

Miguel Valvano

Article

Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

Type

Empty backbone

Expression

Bacterial

Promoter

PrhaB

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRosa26-DEST

Plasmid

#21189

Purpose

Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

Depositor

Nick Hastie, Peter Hohenstein

Article

Hohenstein et al Pathogenetics. 2008 . 1(1):3.

Insert

GateWay conversion cassette

Expression

Mammalian

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-mCherry-DD

Plasmid

#96894

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-mCherry-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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