We narrowed to 16,539 results for: puromycin

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWXLd-RIEP-OgNLuc

   Plasmid

   #135936

   Purpose

   Expresses the OgNLuc LumiFluor in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   OgNLuc LumiFluor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS2-FLAG-Sestrin2-L261A

   Plasmid

   #73678

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

   Insert

   Sesn2 (SESN2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L261A changed Leucine 261 to Alanine

   Promoter

   Sesn2

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pBABE-puro-NLS-LSSmKate1

   Plasmid

   #31873

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS-LSS-mKate1
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRUSH

   Plasmid

   #54480

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLentiCRISPRv1.mir451_KO

   Plasmid

   #164689

   Purpose

   For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   miR-451 CRISPR KO gRNA (MIR451A )
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-puro-CLIP-FLAG-CD59

   Plasmid

   #50375

   Purpose

   Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   CLIP
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMVTCRa_candidate5

   Plasmid

   #164999

   Purpose

   Expression of candidate TCR #5 TCRalpha cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   TRAV (TRA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMVTCRb_candidate5

    Plasmid

    #165000

    Purpose

    Expression of candidate TCR #5 TCRbeta cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    TRB (TRB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOZ-Pax3-FH-C-puro

    Plasmid

    #32517

    Depositor

    Andrew Lassar

    Article

    Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

    Insert

    Pax3 (Pax3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. WT TXNIP-FLAG

    Plasmid

    #200091

    Purpose

    WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-STK3_IDG-K

    Plasmid

    #189879

    Purpose

    Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    STK3 (STK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroSuper-shYes

    Plasmid

    #26987

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Yes (YES1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

15. p663-UBC-miniTurbo-V5-MARK3_IDG-K

    Plasmid

    #170583

    Purpose

    Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MARK3 (MARK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

    Plasmid

    #189903

    Purpose

    Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MAP3K14 (MAP3K14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Drosophila U6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AV11_pDonor.EP.S1

    Plasmid

    #100296

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDSAP

    Plasmid

    #62293

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3486 pBABE Bid p22 GFP

    Plasmid

    #8777

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Science 2000 Dec 1;290(5497):1761-5.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only