We narrowed to 253 results for: T7 rna polymerase

1. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

   Plasmid

   #231353

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

   Plasmid

   #231357

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

   Plasmid

   #231358

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

   Plasmid

   #231359

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRAV23

   Plasmid

   #67715

   Purpose

   Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

   Depositor

   Jeffrey Kieft, Robert Batey

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Transcription cassette for 3'-tagged RNAs
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA polymerase

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRAV24

   Plasmid

   #67716

   Purpose

   Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

   Depositor

   Jeffrey Kieft, Robert Batey

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Transcription cassette for 3'-tagged RNAs
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA polymerase

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAJ1

   Plasmid

   #138379

   Purpose

   pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ

   Depositor

   Seth Darst

   Article

   Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11

   Inserts

   RpoA (rpoA E. coli)
   RpoB (rpoB E. coli)
   RpoC (rpoC E. coli)
   rpoZ (rpoZ E. coli)
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…

   Promoter

   T7

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNG1256

   Plasmid

   #149710

   Purpose

   Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   rpoZ (rpoZ )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAIP4_3V46

   Plasmid

   #234165

   Purpose

   Heterologous protein expression of cell division control protein 73 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3V46 (CDC73 Budding Yeast)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pRAV12

    Plasmid

    #67713

    Purpose

    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

    Depositor

    Jeffrey Kieft, Robert Batey

    Article

    Kieft et al RNA. 2004 Jun;10(6):988-95.

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge