Skip to main content
Addgene
Showing: 801 - 850 of 1002 results

  1. pZ:F3-P CAGGS tdTPH-F

    Plasmid
    #112667
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    112667-plasmid-map-sequence-id-229330

    Enlarge

  2. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

    Enlarge

  3. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

    Enlarge

  4. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177211-plasmid-map-sequence-id-349887

    Enlarge

  8. pCALNL-DsRed

    Plasmid
    #13769
    Purpose
    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    DsRed2
    Use
    Cre/Lox; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13769-plasmid-map-sequence-id-7758

    Enlarge

  9. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177232-plasmid-map-sequence-id-363978

    Enlarge

  10. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

    Enlarge

  11. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid
    #177231
    Purpose
    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgNeo2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177231-plasmid-map-sequence-id-352660

    Enlarge

  12. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

    Plasmid
    #177236
    Purpose
    Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgSnrk
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177236-plasmid-map-sequence-id-352699

    Enlarge

  13. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177234-plasmid-map-sequence-id-352695

    Enlarge

  14. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177233-plasmid-map-sequence-id-352662

    Enlarge

  15. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177230
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177230-plasmid-map-sequence-id-352659

    Enlarge

  16. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177227
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177227-plasmid-map-sequence-id-352658

    Enlarge

  17. bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177229
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177229-plasmid-map-sequence-id-351963

    Enlarge

  18. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177225
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177225-plasmid-map-sequence-id-351956

    Enlarge

  19. bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177226
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177226-plasmid-map-sequence-id-351955

    Enlarge

  20. bU6-sgLkb1_2nd-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177228
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177228-plasmid-map-sequence-id-351921

    Enlarge

  21. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Mutation
    Promoter
    pdx
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

    Enlarge

  22. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61576-plasmid-map-sequence-id-209910

    Enlarge

  25. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

    Plasmid
    #168787
    Purpose
    Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Type
    Empty backbone
    Use
    Cre/Lox and Synthetic Biology; Expression in fila…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168787-plasmid-map-sequence-id-353783

    Enlarge

  26. pGL2399

    Plasmid
    #137051
    Purpose
    pRIB-DiCre DiCre expression from ribosomal locus
    Depositor
    Jeremy Mottram
    Article
    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi
    Inserts
    Split Cre Recombinase Rapamycin Inducible Dimerisation
    Blasticidin-S Deaminase
    Use
    Leishmania ribosomal locus expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137051-plasmid-map-sequence-id-275797

    Enlarge

  27. pQadd2

    Plasmid
    #135662
    Purpose
    Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Lox511/66 and LoxFas/71
    Use
    Clostridial vector, pamβ1 origin
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135662-plasmid-map-sequence-id-305688

    Enlarge

Showing: 801 - 850 of 1002 results