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KHBD00205

Plasmid

#34356

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8068 (Su(var)2-10 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM107

Plasmid

#40153

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SA (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

7SA; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFM103

Plasmid

#40149

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, wild-type (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM109

Plasmid

#40155

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, C56S (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM108

Plasmid

#40154

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, S241A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM106

Plasmid

#40152

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R163A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM105

Plasmid

#40151

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, K162A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM104

Plasmid

#40150

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R183-5A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded …

Promoter

T7

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pFM034

Plasmid

#40137

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (wild-type) (par-2 )

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

pie-1

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
eGFP-proα2(I)-L

Plasmid

#120160

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-proα2(I)-L

Plasmid

#120161

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

mApple

Expression

Mammalian

Mutation

Col1a2 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHM103

Plasmid

#225527

Purpose

Encodes constitutive GFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP505

Plasmid

#200511

Purpose

insertional mutagenesis vector

Depositor

Gregory Pazour

Article

Pazour et al J Cell Biol. 1995 Oct;131(2):427-40. doi

Insert

NIT1

Use

Insertional mutagenesis vector

Tags

Expression

Mutation

unknown

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP

Plasmid

#85426

Purpose

Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_Gal4UAS_humanIL-2_T2A_MIP1α_PGK_mCherry

Plasmid

#85429

Purpose

Lentiviral vector for Gal4 inducible human IL-2/MIP1a with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Human IL-2 T2A MIPalpha

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
Flag‐Tim2

Plasmid

#49213

Purpose

expresses Flag tagged Tim2

Depositor

Lawrence Kane

Article

Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Insert

Tim2 (Timd2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

no Tim2 signal sequence; R80L and S196D compared …

Promoter

EF1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00173

Plasmid

#34326

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7807 (AP-2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only