We narrowed to 31,957 results for: Eng

1. Pvalb-2A-Flpo Flp-in replacement vector

   Plasmid

   #61572

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Flpo
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Snap25-LSL-2A-GFP targeting vector

   Plasmid

   #61575

   Purpose

   Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Snap25-LSL-2A-GFP
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65453

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV1

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   tetO

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV phSyn1(S)-FlpE-bGHpA

   Plasmid

   #50364

   Purpose

   Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   FlpE recombinase gene
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBoBi-hPEN2 D90A

   Plasmid

   #183641

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 90 of PEN2 mutated to alanine

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBoBi-hPEN2 2A

   Plasmid

   #183640

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 35 and 40 of PEN2 mutated to alanine

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65429

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ai62(TITL-tdT) Flp-in replacement vector

   Plasmid

   #61576

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   chromatin insulator flanked TRE-LSL-tdTomato
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rasgrf2-2A-dCre targeting vector

   Plasmid

   #61573

   Purpose

   Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Rasgrf2-2A-dCre (Rasgrf2 Mouse, P1 bacteriophage)
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   March 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBoBi-hGOLGA2-C-PEN2

    Plasmid

    #183649

    Purpose

    Lentiviral expression of a golgi-residential PEN2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 and GOLGA2 (PSENEN, GOLGA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    WT

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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